Document Detail


RNF20 and USP44 regulate stem cell differentiation by modulating H2B monoubiquitylation.
MedLine Citation:
PMID:  22681888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Embryonic stem cells (ESCs) maintain high genomic plasticity, which is essential for their capacity to enter diverse differentiation pathways. Posttranscriptional modifications of chromatin histones play a pivotal role in maintaining this plasticity. We now report that one such modification, monoubiquitylation of histone H2B on lysine 120 (H2Bub1), catalyzed by the E3 ligase RNF20, increases during ESC differentiation and is required for efficient execution of this process. This increase is particularly important for the transcriptional induction of relatively long genes during ESC differentiation. Furthermore, we identify the deubiquitinase USP44 as a negative regulator of H2B ubiquitylation, whose downregulation during ESC differentiation contributes to the increase in H2Bub1. Our findings suggest that optimal ESC differentiation requires dynamic changes in H2B ubiquitylation patterns, which must occur in a timely and well-coordinated manner.
Authors:
Gilad Fuchs; Efrat Shema; Rita Vesterman; Eran Kotler; Zohar Wolchinsky; Sylvia Wilder; Lior Golomb; Ariel Pribluda; Feng Zhang; Mahmood Haj-Yahya; Ester Feldmesser; Ashraf Brik; Xiaochun Yu; Jacob Hanna; Daniel Aberdam; Eytan Domany; Moshe Oren
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular cell     Volume:  46     ISSN:  1097-4164     ISO Abbreviation:  Mol. Cell     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-11     Completed Date:  2012-08-30     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  662-73     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE33214
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics*
Chromatin Assembly and Disassembly
Down-Regulation
Embryonic Stem Cells / cytology*,  metabolism
Endopeptidases / metabolism,  physiology*
Epigenesis, Genetic
Histones / metabolism*
Humans
Mice
Models, Genetic
Ubiquitin-Protein Ligases / metabolism,  physiology*
Ubiquitination
Grant Support
ID/Acronym/Agency:
R37 CA040099/CA/NCI NIH HHS; R37 CA40099/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Histones; EC 3.4.-/Endopeptidases; EC 3.4.99.-/ubiquitin-specific protease; EC 6.3.2.19/RNF20 protein, human; EC 6.3.2.19/RNF20 protein, mouse; EC 6.3.2.19/Ubiquitin-Protein Ligases
Comments/Corrections

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