| RNAi targeting ryanodine receptor 2 protects rat cardiomyocytes from injury caused by simulated ischemia-reperfusion. | |
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MedLine Citation:
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PMID: 20053523 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of a small interfering RNA targeting ryanodine receptor 2 (si-Ryr2) on cardiomyocytes injury following a simulated ischemia-reperfusion (I/R) were investigated. Pretreated with si-Ryr2 or ryanodine, primary cultures of neonatal rat cardiomyocytes were subjected to a protocol of simulated I/R. Compared with control, the cytosolic Ca(2+) concentration ([Ca(2+)](i)) and the generation of reactive oxygen species (ROS) was significantly augmented after I/R. Concomitant with these, cell injury assessed by Annexin V/PI staing, mitochondria membrane potential (DeltaPsim) and the leakage of lactic dehydrogenase (LDH) and creatine phosphokinase (CPK) were aggravated. Si-Ryr2 treatment reduced [Ca(2+)](i) and ROS generation and protected the cardiomyocytes from subsequent I/R injury, as evidenced by stable DeltaPsim and decreased Annexin V(+) PI(-) staing and enzymes release. Moreover, si-Ryr2 exerted more effective protection on I/R injury compared to ryanodine. The present study demonstrated for the first time that in neonatal cardiomyocytes, si-Ryr2 reduces cell death associated with attenuating [Ca(2+)](i) and ROS production. Furthermore, we attempt to speculate that si-Ryr2 excel ryanodine in Ryr2 function research of cardioprotection. |
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Authors:
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Zhuying Guo; Shiting Wang; Qiang Jiao; Manghua Xu; Fenghou Gao |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-18 |
Journal Detail:
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Title: Biomedicine & pharmacotherapy = Biom?decine & pharmacoth?rapie Volume: 64 ISSN: 1950-6007 ISO Abbreviation: Biomed. Pharmacother. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-12 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8213295 Medline TA: Biomed Pharmacother Country: France |
Other Details:
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Languages: eng Pagination: 184-90 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Masson SAS. All rights reserved. |
Affiliation:
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Experimental Center, No. 3 People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine, No. 280 Mohe Road, Baoshan District, Shanghai, China. zyguoo@126.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Calcium Signaling / drug effects*, physiology Carbon Dioxide / pharmacology Cells, Cultured / drug effects Membrane Potential, Mitochondrial / drug effects Mitochondria, Heart / drug effects Myocardial Reperfusion Injury / prevention & control* Myocytes, Cardiac / drug effects* Nitrogen / pharmacology Oxygen / pharmacology RNA Interference* RNA, Small Interfering / pharmacology* Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism Ryanodine / pharmacology Ryanodine Receptor Calcium Release Channel / biosynthesis, drug effects*, genetics, physiology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 0/Reactive Oxygen Species; 0/Ryanodine Receptor Calcium Release Channel; 124-38-9/Carbon Dioxide; 15662-33-6/Ryanodine; 7727-37-9/Nitrogen; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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