| RNAi of the receptor tyrosine phosphatase HmLAR2 in a single cell of an intact leech embryo leads to growth-cone collapse. | |
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MedLine Citation:
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PMID: 10996077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Receptor protein tyrosine phosphatases (RPTPs) are important for growth-cone migration [1-5], but their specific roles have yet to be defined. Previously, we showed that the growth cones of the Comb cell, an embryonic cell in the leech, express high levels of an RPTP called HmLAR2 [6,7]. Here, we report the use of RNA interference (RNAi) to block expression of HmLAR2 in individual Comb cells in the developing embryo. HmLAR2 mRNA levels were reduced in the soma, processes and growth cones of Comb cells injected with double-stranded RNA (dsRNA) for HmLAR2, but no decrease was detected when control dsRNAs were injected. Consistent with this observation, the level of phosphotyrosine increased significantly in the growth cones of Comb cells injected with HmLAR2 dsRNA. Within 24 hours, the growth cones of treated cells showed a distinct collapsed phenotype, with sharp reductions in lamellipodial surface area and in numbers of filopodia. These experiments indicate a key role for LAR-like RPTPs in maintaining the integrity of the growth cone. |
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Authors:
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M W Baker; E R Macagno |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Current biology : CB Volume: 10 ISSN: 0960-9822 ISO Abbreviation: Curr. Biol. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-11-07 Completed Date: 2000-11-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1071-4 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Columbia University, New York, New York 10027, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Embryonic Development* Leeches / embryology*, enzymology Protein Tyrosine Phosphatases / genetics* RNA, Double-Stranded / administration & dosage, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Double-Stranded; EC 3.1.3.-/HmLAR2 protein, Hirudo medicinalis; EC 3.1.3.48/Protein Tyrosine Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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