| RNAi-mediated inhibition of MSP58 decreases tumour growth, migration and invasion in a human glioma cell line. | |
| | |
MedLine Citation:
|
PMID: 18798870 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
MSP58, a 58-kD nuclear microspherule protein, is an evolutionarily conserved nuclear protein implicated in the regulation of gene transcription as well as in malignant transformation. An analysis of mRNA expression by real-time PCR revealed that MSP58 was significantly up-regulated in 29% of high-grade glioblastoma tissues as well as in four glioblastoma cell lines. In the present study, we further evaluated the biological functions of MSP58 in U251 glioma cell proliferation, migration, invasion and tumour growth in vivo by specific MSP58 knockdown using short hairpin RNA (shRNA). We found that MSP58 depletion inhibited glioma cell growth, primarily by inducing cell cycle arrest rather than apoptosis. MSP58 depletion also decreased the invasive capability of glioma cells and anchorage-independent colony formation in soft agar. Moreover, suppression of MSP58 expression significantly impaired the growth of glioma xenografts in nude mice. Finally, a cell cycle-associated gene array revealed potential molecular mechanisms contributing to cell cycle arrest in MSP58-depleted glioma cells. In summary, our data highlight the importance of MSP58 in glioma progression and provided a biological basis for MSP58 as a novel candidate target for treatment of glioma. |
| | |
Authors:
|
Wei Lin; Jing Zhang; Jian Zhang; Xinping Liu; Zhou Fei; Xia Li; Laetitia Davidovic; Zhuo Tang; Lan Shen; Yanchun Deng; Angang Yang; Hua Han; Xiang Zhang; Libo Yao |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular and molecular medicine Volume: 13 ISSN: 1582-4934 ISO Abbreviation: J. Cell. Mol. Med. Publication Date: 2009 Nov-Dec |
Date Detail:
|
Created Date: 2010-04-20 Completed Date: 2010-07-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101083777 Medline TA: J Cell Mol Med Country: England |
Other Details:
|
Languages: eng Pagination: 4608-22 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, The Fourth Military Medical University and The State Key Laboratory of Cancer Biology, Xi'an, The People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Adhesion Cell Cycle / genetics Cell Line, Tumor Cell Movement* Cell Proliferation Down-Regulation / genetics Female Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Glioma / genetics, pathology* Humans Mice Mice, Inbred BALB C Neoplasm Invasiveness Nuclear Proteins / genetics, metabolism* RNA Interference* RNA, Messenger / genetics, metabolism RNA, Small Interfering / metabolism RNA-Binding Proteins / genetics, metabolism* Tumor Stem Cell Assay |
| Chemical | |
Reg. No./Substance:
|
0/MCRS1 protein, human; 0/Nuclear Proteins; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/RNA-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Ubiquitin C-terminal Hydrolase UCH-L1 regulates B-cell proliferation and integrin activation.
Next Document: Extracellular pyridine nucleotides induce PR gene expression and disease resistance in Arabidopsis.