Document Detail


RNAi-mediated blocking of ezrin reduces migration of ectopic endometrial cells in endometriosis.
MedLine Citation:
PMID:  22544491     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Ezrin is a member of the ezrin-radixin-moesin (ERM) family of membrane cytoskeletal linkage proteins. It is important for maintenance of cell shape, adhesion, migration and division. Overexpression of ezrin in some tumours is associated with increased cell migration that is mediated by the Rho/ROCK family of small GTPases. To investigate the role of ezrin in the migration of ectopic endometrial cells in endometriosis, we conducted real time quantitative RT-PCR analysis of eutopic and ectopic endometrium from women with endometriosis compared with endometrium from women without the disease. RNAi, wound healing assays and western blot analysis of endometriotic cells were also included in this research. We found significantly higher levels of mRNA expression of ezrin (0.42 v 0.27, P<0.05), RhoA (0.99 v 0.74, p<0.05), RhoC (0.79 v 0.43, P<0.005) and ROCK1 (0.68 v 0.38, P<0.005) in ectopic endometrial cells compared to eutopic endometrial cells in endometriosis. Blocking ezrin with siRNA reduced migration of ectopic endometrial cells with decreased expression of RhoA (42.68%), RhoC (58.42%), and ROCK1 (59.88%). Our results indicate that overexpression of ezrin in endometriosis may play a significant role in the migration of endometrial cells of endometriosis, and, the RhoC/Rock pathway may provide a promising treatment target.
Authors:
Qiao-Ying Jiang; Jian-Mei Xia; Hai-Gang Ding; Xiang-Wei Fei; Jun Lin; Rui-Jin Wu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-26
Journal Detail:
Title:  Molecular human reproduction     Volume:  -     ISSN:  1460-2407     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
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