| RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells. | |
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MedLine Citation:
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PMID: 20874842 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ecto-5'-nucleotidase (CD73), a cell surface protein that hydrolyzes extracellular AMP into adenosine and phosphate, is overexpressed in many solid tumors. In this study, we tested the hypothesis that increased CD73 may promote tumor progression by examining the effect of CD73 suppression via RNA interference and CD73 overexpression on tumor growth in vivo and in vitro. Using digitized whole-body images, plate clone forming assay and TUNEL assay in frozen tissue sections, we found that the cell growth rate was significantly lower in vivo and in vitro after CD73 suppression and late apoptosis was much higher in xenograft tumors developed from the CD73-siRNA transfected MB-MDA-231 clone (P1). By flow cytometry, the P1 cell cycle was arrested in the G0/G1 phase. Moreover, Bcl-2 was downregulated, while Bax and caspase-3 were upregulated with CD73 suppression. CD73 inhibitor α,β-methylene adenosine-5'-disphosphate (APCP) functioned similarly with RNAi-mediated CD73 suppression. In addition, in transfected MCF-7 cells, we found that CD73 overexpression increased cell viability and promoted cell cycle progression, depending on its enzyme activity. More intriguingly, CD73 overexpression in MCF-7 breast cancer cells produces a tumorigenic phenotype. We conclude that CD73 plays an important role in breast cancer growth by affecting cell cycle progression and apoptosis. |
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Authors:
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Xiuling Zhi; Yingjian Wang; Xuerui Zhou; Jerry Yu; Rongrong Jian; Shaoxian Tang; Lianhua Yin; Ping Zhou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-28 |
Journal Detail:
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Title: Cancer science Volume: 101 ISSN: 1349-7006 ISO Abbreviation: Cancer Sci. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-16 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101168776 Medline TA: Cancer Sci Country: England |
Other Details:
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Languages: eng Pagination: 2561-9 Citation Subset: IM |
Copyright Information:
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© 2010 Japanese Cancer Association. |
Affiliation:
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Department of Physiology and Pathophysiology, Shanghai Medical College, Shanghai, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5'-Nucleotidase
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biosynthesis*,
genetics Animals Apoptosis / physiology* Blotting, Western Breast Neoplasms / genetics, metabolism* Cell Cycle / physiology* Cell Line, Tumor Cell Separation Female Flow Cytometry Humans Immunohistochemistry In Situ Nick-End Labeling Mice Mice, Nude RNA, Small Interfering / genetics* Reverse Transcriptase Polymerase Chain Reaction Tissue Array Analysis Transfection |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; EC 3.1.3.5/5'-Nucleotidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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