| RNAi-Based Methods for Gene Silencing in Mouse Oocytes. | |
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MedLine Citation:
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PMID: 23138949 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes. |
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Authors:
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Paula Stein; Petr Svoboda; Richard M Schultz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 957 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 135-51 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Pennsylvania, Philadelphia, PA, USA, steinpau@sas.upenn.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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