| RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal. | |
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MedLine Citation:
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PMID: 21543563 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection. |
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Authors:
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K Kébé; O Ndiaye; H Diop Ndiaye; P Mbakob Mengue; P M M Guindo; S Diallo; N Léye; S B Gueye; A Gaye Diallo; C Touré Kane; S Mboup |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2011-05-04 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 49 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-07-01 Completed Date: 2011-09-26 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2590-3 Citation Subset: IM |
Affiliation:
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Laboratoire de Bactériologie Virologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop, Dakar, Senegal. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Viral
/
blood,
genetics,
isolation & purification* Desiccation Early Diagnosis HIV Infections / diagnosis* HIV-1 / genetics, isolation & purification* Humans Infant Infant, Newborn Molecular Diagnostic Techniques / methods* RNA, Viral / blood, genetics, isolation & purification* Reagent Kits, Diagnostic* Senegal Sensitivity and Specificity Specimen Handling / methods Virology / methods* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Viral; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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