Document Detail


RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.
MedLine Citation:
PMID:  21543563     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection.
Authors:
K Kébé; O Ndiaye; H Diop Ndiaye; P Mbakob Mengue; P M M Guindo; S Diallo; N Léye; S B Gueye; A Gaye Diallo; C Touré Kane; S Mboup
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-04
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  49     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-01     Completed Date:  2011-09-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2590-3     Citation Subset:  IM    
Affiliation:
Laboratoire de Bactériologie Virologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop, Dakar, Senegal.
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MeSH Terms
Descriptor/Qualifier:
DNA, Viral / blood,  genetics,  isolation & purification*
Desiccation
Early Diagnosis
HIV Infections / diagnosis*
HIV-1 / genetics,  isolation & purification*
Humans
Infant
Infant, Newborn
Molecular Diagnostic Techniques / methods*
RNA, Viral / blood,  genetics,  isolation & purification*
Reagent Kits, Diagnostic*
Senegal
Sensitivity and Specificity
Specimen Handling / methods
Virology / methods*
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral; 0/Reagent Kits, Diagnostic
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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