Document Detail

RNA trafficking signals in human immunodeficiency virus type 1.
MedLine Citation:
PMID:  11238947     Owner:  NLM     Status:  MEDLINE    
Intracellular trafficking of retroviral RNAs is a potential mechanism to target viral gene expression to specific regions of infected cells. Here we show that the human immunodeficiency virus type 1 (HIV-1) genome contains two sequences similar to the hnRNP A2 response element (A2RE), a cis-acting RNA trafficking sequence that binds to the trans-acting trafficking factor, hnRNP A2, and mediates a specific RNA trafficking pathway characterized extensively in oligodendrocytes. The two HIV-1 sequences, designated A2RE-1, within the major homology region of the gag gene, and A2RE-2, in a region of overlap between the vpr and tat genes, both bind to hnRNP A2 in vitro and are necessary and sufficient for RNA transport in oligodendrocytes in vivo. A single base change (A8G) in either sequence reduces hnRNP A2 binding and, in the case of A2RE-2, inhibits RNA transport. A2RE-mediated RNA transport is microtubule and hnRNP A2 dependent. Differentially labelled gag and vpr RNAs, containing A2RE-1 and A2RE-2, respectively, coassemble into the same RNA trafficking granules and are cotransported to the periphery of the cell. tat RNA, although it contains A2RE-2, is not transported as efficiently as vpr RNA. An A2RE/hnRNP A2-mediated trafficking pathway for HIV RNA is proposed, and the role of RNA trafficking in targeting HIV gene expression is discussed.
A J Mouland; H Xu; H Cui; W Krueger; T P Munro; M Prasol; J Mercier; D Rekosh; R Smith; E Barbarese; E A Cohen; J H Carson
Related Documents :
9656387 - Maternal and viral factors in vertical transmission of hiv-1 subtype e.
24788337 - Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of ...
7759117 - The eukaryotic cofactor for the human immunodeficiency virus type 1 (hiv-1) rev protein...
23307607 - Isolation and characterisation of a ferrirhodin synthetase gene from the sugarcane path...
16857237 - Antiandrogenic activity of pyrethroid pesticides and their metabolite in reporter gene ...
20004087 - A gmm-ig framework for selecting genes as expression panel biomarkers.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  21     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-04-19     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2133-43     Citation Subset:  IM    
Laboratory of Human Retrovirology, Department of Microbiology and Immunology, University of Montreal, Montreal, Quebec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Biological Transport
Cells, Cultured
Gene Products, gag / genetics
Gene Products, vpr / genetics
HIV-1 / genetics*,  pathogenicity
Heterogeneous-Nuclear Ribonucleoprotein Group A-B*
Heterogeneous-Nuclear Ribonucleoproteins
Molecular Biology / methods
Molecular Sequence Data
Oligodendroglia / cytology,  virology*
Peptide Fragments / genetics
RNA, Viral / analysis*,  metabolism*
Response Elements / genetics*
Ribonucleoproteins / genetics
vpr Gene Products, Human Immunodeficiency Virus
Grant Support
Reg. No./Substance:
0/Gene Products, gag; 0/Gene Products, vpr; 0/Heterogeneous-Nuclear Ribonucleoprotein Group A-B; 0/Heterogeneous-Nuclear Ribonucleoproteins; 0/Peptide Fragments; 0/RNA, Viral; 0/Ribonucleoproteins; 0/hnRNP A2; 0/vpr Gene Products, Human Immunodeficiency Virus; 0/vpr peptide (13-33), Human immunodeficiency virus 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  beta(3)A-integrin downregulates the urokinase-type plasminogen activator receptor (u-PAR) through a ...
Next Document:  Dual inactivation of RB and p53 pathways in RAS-induced melanomas.