Document Detail

RNA synthesized in calicivirus-infected cells is atypical of picornaviruses.
MedLine Citation:
PMID:  559106     Owner:  NLM     Status:  MEDLINE    
RNA labeled with [3H]uridine from Vero cells infected with San Miguel sea lion virus in the presence of actinomycin D was analyzed by glycerol density gradient sedimentation and polyacrylamide gel electrophoresis. The predominant single-stranded RNA (36S, 2.6 x 10(6) molecular weight) was genome size. There was also a prominent 22S, 1.1 x 10(6)-molecular weight, single-stranded component and one or more double-stranded or partially double-stranded classes. Replicative forms, sedimenting at 18S, contained single-stranded RNA corresponding to the larger-molecular-weight class. All classes of intracellular RNA and virion RNA were polyadenylated. These findings and results with pig kidney cells infected with vesicular exanthema of swine virus and feline cells infected with feline calicivirus indicate that caliciviruses exhibit a strategy of replication different from typical picornaviruses and supports removal of the caliciviruses from the family Picornaviridae.
D W Ehresmann; F L Schaffer
Related Documents :
2985806 - Characterization of a variant virus selected in rat brains after infection by coronavir...
8263116 - In vitro destabilization of plant viruses and cdna synthesis.
3346946 - Molecular analysis of viral rnas in mice persistently infected with lymphocytic choriom...
3016986 - Transcriptional mapping of early rna from regions of the shope fibroma and malignant ra...
23709466 - Distribution, prevalence, and genetic analysis of panulirus argus virus 1 (pav1) from t...
19442836 - Pcr detection of african horse sickness virus serogroup based on genome segment three s...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  22     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  572-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Dactinomycin / pharmacology
Molecular Weight
Picornaviridae / analysis*,  classification,  metabolism
Poly A / analysis
RNA, Viral / analysis*,  biosynthesis
Reg. No./Substance:
0/RNA, Viral; 24937-83-5/Poly A; 50-76-0/Dactinomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Virus-directed post-translational cleavage in Sindbus virus-infected cells.
Next Document:  Contagious ecthyma in mountain goat of coastal British Columbia.