Document Detail


RNA synthesized in calicivirus-infected cells is atypical of picornaviruses.
MedLine Citation:
PMID:  559106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RNA labeled with [3H]uridine from Vero cells infected with San Miguel sea lion virus in the presence of actinomycin D was analyzed by glycerol density gradient sedimentation and polyacrylamide gel electrophoresis. The predominant single-stranded RNA (36S, 2.6 x 10(6) molecular weight) was genome size. There was also a prominent 22S, 1.1 x 10(6)-molecular weight, single-stranded component and one or more double-stranded or partially double-stranded classes. Replicative forms, sedimenting at 18S, contained single-stranded RNA corresponding to the larger-molecular-weight class. All classes of intracellular RNA and virion RNA were polyadenylated. These findings and results with pig kidney cells infected with vesicular exanthema of swine virus and feline cells infected with feline calicivirus indicate that caliciviruses exhibit a strategy of replication different from typical picornaviruses and supports removal of the caliciviruses from the family Picornaviridae.
Authors:
D W Ehresmann; F L Schaffer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  22     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  572-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Dactinomycin / pharmacology
Molecular Weight
Picornaviridae / analysis*,  classification,  metabolism
Pinnipedia
Poly A / analysis
RNA, Viral / analysis*,  biosynthesis
Chemical
Reg. No./Substance:
0/RNA, Viral; 24937-83-5/Poly A; 50-76-0/Dactinomycin
Comments/Corrections

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