| RNA silencing of lactate dehydrogenase gene in Rhizopus oryzae. | |
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MedLine Citation:
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PMID: 21769297 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Rhizopus oryzae is a filamentous fungus, belonging to the order Mucorales. It can ferment a wide range of carbohydrates hydrolyzed from lignocellulosic materials and even cellobiose to produce ethanol. However, R. oryzae also produces lactic acid as a major metabolite, which reduces the yield of ethanol. In this study, we show that significant reduction of lactic acid production could be achieved by short (25nt) synthetic siRNAs targeting the ldhA gene. The average yield of lactic acid production by R. oryzae during the batch fermentation process, where glucose had been used as a sole carbon source, diminished from 0.07gm/gm in wild type to 0.01gm/gm in silenced samples. In contrast, the average yield of ethanol production increased from 0.39gm/gm in wild type to 0.45gm/gm in silenced samples. These results show 85.7% (gm/gm) reduction in lactic acid production as compared with the wild type R. oryzae, while an increase of 15.4% (gm/gm) in ethanol yield. |
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Authors:
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Ali Hashemi Gheinani; Neda Haghayegh Jahromi; Elisabeth Feuk-Lagerstedt; Mohammad J Taherzadeh |
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Publication Detail:
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Type: Journal Article Date: 2011-06-20 |
Journal Detail:
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Title: Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research Volume: 7 ISSN: 1747-0854 ISO Abbreviation: J RNAi Gene Silencing Publication Date: 2011 |
Date Detail:
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Created Date: 2011-07-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101256512 Medline TA: J RNAi Gene Silencing Country: England |
Other Details:
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Languages: eng Pagination: 443-8 Citation Subset: - |
Affiliation:
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University of Borås, School of Engineering, SE-501 90 Borås, Sweden. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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