Document Detail

RNA processing is a limiting step for murine tumor necrosis factor beta expression in response to interleukin-2.
MedLine Citation:
PMID:  1700275     Owner:  NLM     Status:  MEDLINE    
We have previously reported that tumor necrosis factor beta (TNF beta) expression is induced by interleukin-2 (IL-2) in the murine lymphocytic T-cell line CTLL-2. In this study, we have characterized the nuclear and cytoplasmic TNF beta transcript and assessed their role in TNF beta gene expression. A unique feature of TNF beta expression was the accumulation of nuclear precursors, which reflected a slow nuclear RNA processing. As a consequence, there was a delay in the appearance of cytoplasmic messengers after the transcriptional induction of TNF beta by IL-2. We also found that two messengers, the fully spliced messenger and an intron 3-retaining messenger, were exported to the cytoplasm and actively translated. The same pattern of expression was observed in concanavalin A-stimulated splenocytes, although the level of expression was much lower than in CTLL-2 cells. The simple genetic structure and the high level of accumulation of nuclear precursors make TNF beta a particularly attractive model system to use for studies of RNA processing and cytoplasmic transport of partially spliced messengers.
D Weil; S Brosset; F Dautry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  10     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-05     Completed Date:  1990-12-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5865-75     Citation Subset:  IM    
Laboratoire d'Oncologie Moléculaire, Institut Gustave Roussy, Centre National de la Recherche Scientifique UA 1158, Villejuif, France.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cell Line
Cell Nucleus / metabolism
Concanavalin A / pharmacology
Cytoplasm / metabolism
Gene Library
Interleukin-2 / pharmacology*
Lymphocyte Activation
Lymphotoxin-alpha / genetics*
Mice, Inbred BALB C
Molecular Sequence Data
Protein Biosynthesis
RNA / genetics,  isolation & purification
RNA, Messenger / genetics*,  metabolism
T-Lymphocytes, Cytotoxic
Transcription, Genetic*
Reg. No./Substance:
0/Interleukin-2; 0/Lymphotoxin-alpha; 0/RNA, Messenger; 11028-71-0/Concanavalin A; 63231-63-0/RNA

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