Document Detail

RNA polymerase II interacts with the promoter region of the noninduced hsp70 gene in Drosophila melanogaster cells.
MedLine Citation:
PMID:  3099167     Owner:  NLM     Status:  MEDLINE    
By using a protein-DNA cross-linking method (D. S. Gilmour and J. T. Lis, Mol. Cell. Biol. 5:2009-2018, 1985), we examined the in vivo distribution of RNA polymerase II on the hsp70 heat shock gene in Drosophila melanogaster Schneider line 2 cells. In heat shock-induced cells, a high level of RNA polymerase II was detected on the entire gene, while in noninduced cells, the RNA polymerase II was confined to the 5' end of the hsp70 gene, predominantly between nucleotides -12 and +65 relative to the start of transcription. This association of RNA polymerase II was apparent whether the cross-linking was performed by a 10-min UV irradiation of chilled cells with mercury vapor lamps or by a 40-microsecond irradiation of cells with a high-energy xenon flash lamp. We hypothesize that RNA polymerase II has access to, and a high affinity for, the promoter region of this gene before induction, and this poised RNA polymerase II may be critical in the mechanism of transcription activation.
D S Gilmour; J T Lis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  6     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-02-17     Completed Date:  1987-02-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3984-9     Citation Subset:  IM    
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MeSH Terms
DNA / metabolism
Drosophila melanogaster / genetics*
Genes* / radiation effects
Heat-Shock Proteins / biosynthesis,  genetics*
Promoter Regions, Genetic*
Protein Binding
RNA Polymerase II / metabolism*
Ultraviolet Rays
Grant Support
Reg. No./Substance:
0/Heat-Shock Proteins; 9007-49-2/DNA; EC 2.7.7.-/RNA Polymerase II

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