Document Detail


RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei.
MedLine Citation:
PMID:  19165144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the protozoan parasite Trypanosoma brucei, the two main surface glycoprotein genes are transcribed by RNA polymerase I (pol I) instead of RNA pol II, the polymerase committed to the production of mRNA in eukaryotes. This unusual feature might be accomplished by the recruitment of specific subunits or cofactors that allow pol I to transcribe protein-coding RNAs. Here, we report that transcription mediated by pol I requires TbRPB7, a dissociable subunit of the pol II complex. TbRPB7 was found to interact with two pol I-specific subunits, TbRPA1 and TbRPB6z. Pol I-specific transcription was affected on depletion of TbRPB7 in run-on assays, whereas recombinant TbRPB7 increased transcription driven by a pol I promoter. These results represent a unique example of a functional RNA polymerase chimaera consisting of a core pol I complex that recruits a specific pol II subunit.
Authors:
Xenia Peñate; Diana López-Farfán; David Landeira; Amy Wentland; Isabel Vidal; Miguel Navarro
Related Documents :
23500534 - Differential regulation of wnt/beta-catenin signaling by liver x receptors in schwann c...
9642284 - Regulation of a tata-binding protein-associated factor during cellular differentiation.
23776394 - Altered app carboxyl-terminal processing under ferrous iron treatment in pc12 cells.
25086674 - The expression levels and prognostic value of high temperature required a2 (htra2) in n...
23142464 - Fluorochemicals used in food packaging inhibit male sex hormone synthesis.
12392994 - The basic-helix-loop-helix transcription factor hand2 directly regulates transcription ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-23
Journal Detail:
Title:  EMBO reports     Volume:  10     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-03     Completed Date:  2009-06-29     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  252-7     Citation Subset:  IM    
Affiliation:
Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas, CSIC (Spanish National Research Council), Avda. del Conocimiento s/n, 18100 Granada, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / metabolism
Genes, Reporter
Protein Subunits / genetics,  metabolism*
Protozoan Proteins / genetics,  metabolism*
RNA Interference
RNA Polymerase I / genetics,  metabolism*
RNA Polymerase II / chemistry,  genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Transcription, Genetic*
Trypanosoma brucei brucei / cytology,  enzymology*,  genetics
Grant Support
ID/Acronym/Agency:
HHMI-55005525//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Protein Subunits; 0/Protozoan Proteins; 0/Recombinant Fusion Proteins; EC 2.7.7.-/RNA Polymerase I; EC 2.7.7.-/RNA Polymerase II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Strep...
Next Document:  BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation.