Document Detail

RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei.
MedLine Citation:
PMID:  19165144     Owner:  NLM     Status:  MEDLINE    
In the protozoan parasite Trypanosoma brucei, the two main surface glycoprotein genes are transcribed by RNA polymerase I (pol I) instead of RNA pol II, the polymerase committed to the production of mRNA in eukaryotes. This unusual feature might be accomplished by the recruitment of specific subunits or cofactors that allow pol I to transcribe protein-coding RNAs. Here, we report that transcription mediated by pol I requires TbRPB7, a dissociable subunit of the pol II complex. TbRPB7 was found to interact with two pol I-specific subunits, TbRPA1 and TbRPB6z. Pol I-specific transcription was affected on depletion of TbRPB7 in run-on assays, whereas recombinant TbRPB7 increased transcription driven by a pol I promoter. These results represent a unique example of a functional RNA polymerase chimaera consisting of a core pol I complex that recruits a specific pol II subunit.
Xenia Peñate; Diana López-Farfán; David Landeira; Amy Wentland; Isabel Vidal; Miguel Navarro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-23
Journal Detail:
Title:  EMBO reports     Volume:  10     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-03     Completed Date:  2009-06-29     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  252-7     Citation Subset:  IM    
Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas, CSIC (Spanish National Research Council), Avda. del Conocimiento s/n, 18100 Granada, Spain.
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MeSH Terms
Cell Nucleus / metabolism
Genes, Reporter
Protein Subunits / genetics,  metabolism*
Protozoan Proteins / genetics,  metabolism*
RNA Interference
RNA Polymerase I / genetics,  metabolism*
RNA Polymerase II / chemistry,  genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Transcription, Genetic*
Trypanosoma brucei brucei / cytology,  enzymology*,  genetics
Grant Support
HHMI-55005525//Howard Hughes Medical Institute
Reg. No./Substance:
0/Protein Subunits; 0/Protozoan Proteins; 0/Recombinant Fusion Proteins; EC 2.7.7.-/RNA Polymerase I; EC 2.7.7.-/RNA Polymerase II

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