Document Detail


RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.
MedLine Citation:
PMID:  18023414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
tNOX, a tumor-associated NADH oxidase, is a growth-related protein present in transformed cells. In this study, we employed RNA interference (RNAi)-mediated down-regulation of tNOX protein expression to explore the role of tNOX in regulating cell growth in human cervical adenocarcinoma (HeLa) cells. In this first reported use of RNAi to decrease tNOX expression, we found that HeLa cell growth was significantly inhibited by shRNA-knockdown of tNOX. Furthermore, cell migration and membrane association of Rac were decreased concomitantly with the reduction in tNOX protein expression. These results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. We propose that tNOX is a potential upstream mediator of Rho activation that plays a role in regulating cell proliferation, migration, and invasion.
Authors:
Shan-Chi Liu; Jaw-Ji Yang; Kuo-Ning Shao; Pin Ju Chueh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-20
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  365     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  672-7     Citation Subset:  IM    
Affiliation:
Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / metabolism*
Cell Movement*
Gene Silencing
Gene Targeting / methods*
Hela Cells
Humans
NADH, NADPH Oxidoreductases / genetics,  metabolism*
RNA Interference*
Chemical
Reg. No./Substance:
EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.-/tumor-associated NADH oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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