Document Detail

RNA interference suggests sulfakinins as satiety effectors in the cricket Gryllus bimaculatus.
MedLine Citation:
PMID:  17560597     Owner:  NLM     Status:  MEDLINE    
In the Mediterranean field cricket, Gryllus bimaculatus, the action of sulfakinin (SK) gene expression on food intake, food transport in the gut and carbohydrate digestion (alpha-amylase activity) was investigated by using the RNA interference (RNAi) method. Injection of SK double-stranded (ds) RNA into the abdomen of female adults and last instar larvae led to a systemic silencing of the SK gene, as was shown by RT-PCR studies. In adults, suppression of SK gene expression was effective from the first day after injection up to at least the third day. Treatment of the adult crickets by injection or feeding of dsRNA led to a stimulation of the food intake. Assuming that the gene silencing is followed by a depletion of the SK in tissues and/or haemolymph implies an inhibitiory role of the native SK peptides on food intake. The alpha-amylase activity in vitro in the midgut tissue and in the secretions of adult females was not affected by silencing the SK gene.
Martina Meyering-Vos; Adrienne Müller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-24
Journal Detail:
Title:  Journal of insect physiology     Volume:  53     ISSN:  0022-1910     ISO Abbreviation:  J. Insect Physiol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-13     Completed Date:  2007-11-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985080R     Medline TA:  J Insect Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  840-8     Citation Subset:  IM    
Department of Animal Ecology I, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany.
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MeSH Terms
Eating / physiology*
Feeding Behavior / physiology*
Gastrointestinal Tract / metabolism,  physiology
Gene Expression
Gryllidae / genetics,  metabolism*
Neuropeptides / genetics,  metabolism*
RNA Interference
RNA, Double-Stranded
alpha-Amylases / metabolism*
Reg. No./Substance:
0/Neuropeptides; 0/RNA, Double-Stranded; EC

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