| RNA interference of the period gene affects the rhythm of sperm release in moths. | |
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MedLine Citation:
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PMID: 19150927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The period (per) gene is 1 of the core elements of the circadian clock mechanism in animals from insects to mammals. In clock cells of Drosophila melanogaster, per mRNA and PER protein oscillate in daily cycles. Consistent with the molecular clock model, PER moves to cell nuclei and acts as a repressor of positive clock elements. Homologs of per are known in many insects; however, specific roles of per in generating output rhythms are not known for most species. The aim of this article was to determine whether per is functionally involved in the circadian rhythm of sperm release in the moth, Spodoptera littoralis. In this species, as in other moths, rhythmic release of sperm bundles from the testis into the upper vas deferens occurs only in the evening, and this rhythm continues in the isolated reproductive system. S. littoralis was used to investigate the expression of per mRNA and protein in the 2 types of cells involved in sperm release: the cyst cells surrounding sperm bundles in the testes, and the barrier cells separating testicular follicles from the vas deferens. In cyst cells, PER showed a nuclear rhythm in light/dark (LD) cycles but was constitutively cytoplasmic in constant darkness (DD). In barrier cells, nuclear cycling of PER was observed in both LD and DD. To determine the role of PER in rhythmic sperm release in moths, testes-sperm duct complexes were treated in vitro with double-stranded fragments of per mRNA (dsRNA). This treatment significantly lowered per mRNA and protein in cyst cells and barrier cells and caused a delay of sperm release. These data demonstrate that a molecular oscillator involving the period gene plays an essential role in the regulation of rhythmic sperm release in this species. |
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Authors:
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Joanna Kotwica; Piotr Bebas; Barbara O Gvakharia; Jadwiga M Giebultowicz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of biological rhythms Volume: 24 ISSN: 0748-7304 ISO Abbreviation: J. Biol. Rhythms Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-19 Completed Date: 2009-04-02 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8700115 Medline TA: J Biol Rhythms Country: United States |
Other Details:
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Languages: eng Pagination: 25-34 Citation Subset: IM |
Affiliation:
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Department of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Circadian Rhythm* Darkness Drosophila Proteins Gene Expression Regulation Immunohistochemistry / methods Light Male Models, Biological Moths Nuclear Proteins / genetics*, physiology* Period Circadian Proteins RNA Interference* RNA, Double-Stranded / metabolism RNA, Messenger / metabolism Spermatozoa / physiology* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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GM073792/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Nuclear Proteins; 0/PER protein, Drosophila; 0/Period Circadian Proteins; 0/RNA, Double-Stranded; 0/RNA, Messenger |
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