Document Detail


RNA interference for discoidin domain receptor 2 attenuates neointimal formation in balloon injured rat carotid artery.
MedLine Citation:
PMID:  18497308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Discoidin domain receptor 2 (DDR2) plays potential roles in the regulation of collagen turnover mediated by smooth muscle cells (SMCs) in atherosclerosis. Little is known about the function of DDR2 in vascular system. We investigated whether inhibition of DDR2 by small interfering RNA (siRNA) can reduce neointimal formation after arterial injury.
METHODS AND RESULTS: SMCs from thoracic aorta of adult Wistar rats were cultured. The carotid artery from adult Wistar rats was injured by balloon catheter. DDR2 significantly increased migration and proliferation of SMCs. DDR2 siRNA inhibited 86% of DDR2 protein expression in cultured SMCs. DDR2 protein and mRNA expression significantly increased at 14 days after carotid injury. DDR2 siRNA significantly reduced DDR2 protein and mRNA expression induced by balloon injury. The immunohistochemical stain demonstrated that DDR2 siRNA decreased MMP2 protein labeling induced by balloon injury, a pattern similar to that of DDR2 protein labeling. Neointimal area was significantly increased after carotid injury and was significantly reduced by DDR2 siRNA.
CONCLUSIONS: DDR2 increases migration and proliferation of SMCs, and expression of DDR2 in carotid artery significantly increases after injury. DDR2 siRNA attenuates neointimal formation after carotid injury. DDR2 may play a pivotal role in the pathogenesis of neointimal thickening after mechanical injury.
Authors:
Kou-Gi Shyu; Bao-Wei Wang; Peiliang Kuan; Hang Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-22
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  28     ISSN:  1524-4636     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-24     Completed Date:  2008-09-25     Revised Date:  2012-06-01    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1447-53     Citation Subset:  IM    
Affiliation:
Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, 95 Wen-Chang Rd, Taipei, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Thoracic / cytology,  physiopathology*
Carotid Artery Injuries / physiopathology*
Cells, Cultured
Disease Models, Animal
Muscle, Smooth, Vascular / physiology,  physiopathology*
Myocytes, Smooth Muscle / physiology
RNA Interference
RNA, Small Interfering
Rats
Rats, Wistar
Receptor Protein-Tyrosine Kinases / genetics,  physiology*
Receptors, Mitogen / genetics,  physiology*
Tunica Intima / cytology,  physiopathology*
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; 0/Receptors, Mitogen; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.1/discoidin receptor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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