Document Detail


The RNA interference of amino acid transporter LAT1 inhibits the growth of KB human oral cancer cells.
MedLine Citation:
PMID:  16886618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Amino acid transporters are essential for growth and proliferation in all living cells. Among the amino acid transporters, the system L amino acid transporters are the major nutrient transport system responsible for the Na+-independent transport of neutral amino acids, including several essential amino acids. The L-type amino acid transporter 1 (LAT1) is overexpressed to support cell growth in malignant tumors. Double-stranded RNA-mediated RNA interference (RNAi) analysis can be used in a wide variety of eukaryotes to induce the sequence-specific inhibition of gene expression. The current study attempted to investigate the effects of silencing LAT1 expression with small interfering RNA (siRNA) on cell growth in the KB human oral squamous cell carcinoma. MATERIALS AND METHODS: The effects of silencing LAT1 expression with siRNA KB on cell growth were examined using RT-PCR, Western blot analysis, amino acid transport measurement and the MTT assay. RESULTS: In the RT-PCR and Western blot analyses, the siRNA of LAT1 inhibited the expressions of LAT1 mRNA and protein. The uptake of [14C]L-leucine was also inhibited by the siRNA of LAT1. In the MTT assay, the siRNA of LAT1 inhibited the growth of the KB cells in a time-dependent manner, indicating that this growth inhibition was induced by the LAT1-mediated blocking of neutral amino acid transport. CONCLUSION: The transport of neutral amino acids, including several essential amino acids, into the KB human oral squamous cell carcinoma is mainly mediated by LAT1. Furthermore, LAT1 could be a new target for the inhibition of cancer cell growth.
Authors:
Chang-Hyun Kim; Kyung Jin Park; Jung Rim Park; Yoshikatsu Kanai; Hitoshi Endou; Joo-Cheol Park; Do Kyung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  26     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2006 Jul-Aug
Date Detail:
Created Date:  2006-08-04     Completed Date:  2006-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2943-8     Citation Subset:  IM    
Affiliation:
Oral Biology Research Institute, Chosun University College of Dentistry, Gwangju 501-759, Korea.
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MeSH Terms
Descriptor/Qualifier:
Carbon Radioisotopes
Carcinoma, Squamous Cell / genetics,  metabolism
Cell Growth Processes / genetics
Gene Silencing
Humans
KB Cells
Large Neutral Amino Acid-Transporter 1 / biosynthesis,  genetics*,  metabolism*
Leucine / metabolism,  pharmacokinetics
Mouth Neoplasms / genetics,  metabolism
RNA Interference
RNA, Small Interfering / genetics
Transfection
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Large Neutral Amino Acid-Transporter 1; 0/RNA, Small Interfering; 61-90-5/Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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