| RNA interference of WdFKS1 mRNA expression causes slowed growth, incomplete septation and loss of cell wall integrity in yeast cells of the polymorphic, pathogenic fungus Wangiella (Exophiala) dermatitidis. | |
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MedLine Citation:
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PMID: 21469912 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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As one of the main components of the fungal cell wall, β-1,3-glucan provides the mechanical strength to protect fungal protoplasts. The enzyme responsible for the synthesis of β-1,3-glucans in fungi is β-1,3-glucan synthase. Here we report the cloning, sequencing and characterization of the WdFKS1 gene, which in the pathogenic fungus Wangiella dermatitidis encodes the catalytic domain of its β-1, 3-glucan synthase. Because our research suggested that WdFKS1 is a single copy essential gene, we used RNA interference to reduce its expression. Reduction of the WdFKS1 messenger retarded growth and caused the loss of cell wall integrity of yeast cells, but not hyphae or sclerotic cells. We suggest that the WdFKS1 in this polymorphic agent of phaeohyphomycosis is not only required for cell wall construction and maintenance, but also is involved in septum formation. |
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Authors:
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Pengfei Guo; Paul J Szaniszlo |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-6 |
Journal Detail:
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Title: Medical mycology : official publication of the International Society for Human and Animal Mycology Volume: - ISSN: 1460-2709 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815835 Medline TA: Med Mycol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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The Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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