Document Detail


RNA-containing exosomes in human nasal secretions.
MedLine Citation:
PMID:  21172122     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Exosomes are nanovesicles of endocytic origin released by cells and present in human body fluids such as plasma, breast milk, and bronchoalveolar lavage fluid. These vesicles take part in communication between cells. Recently, it was shown that exosomes contain both mRNA and microRNA. This RNA can be shuttled between cells (exosomal shuttle RNA), which is a new route of communication between cells. The aim of this study was to determine whether nasal secretions harbor exosomes and furthermore, whether these exosomes contain RNA.
METHODS: Human nasal lavage fluid (NLF) underwent centrifugation and filtration to discard cells and debris, followed by a final ultracentrifugation at 120,000 × g to pellet the exosomes. Exosomes were detected using electron microscopy (EM), flow cytometry, and Western blot. RNA was extracted and analyzed using a Bioanalyzer.
RESULTS: Exosomes were visualized as 40-80 nm, CD63(+) vesicles using EM. Flow cytometry of exosomes using anti-major histocompatibility complex class II beads revealed exosomes positive for the tetraspanins CD9, CD63, and CD81. Western blot confirmed the presence of exosomal protein and absence of proteins from the endoplasmic reticulum (ER), because the exosomes were positive for Tsg101, but negative for the ER marker, calnexin. Bioanalyzer analysis revealed that, these exosomes contain RNA.
CONCLUSION: This study shows for the first time that NLF contains exosomes and that these exosomes contain RNA. Further characterization of the exosomal RNA and proteins may provide important information about communication in the nose and potentially provide a source of biomarkers for upper airway diseases.
Authors:
Cecilia Lässer; Serena E O'Neil; Linda Ekerljung; Karin Ekström; Margareta Sjöstrand; Jan Lötvall
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-17
Journal Detail:
Title:  American journal of rhinology & allergy     Volume:  25     ISSN:  1945-8932     ISO Abbreviation:  Am J Rhinol Allergy     Publication Date:    2011 Mar-Apr
Date Detail:
Created Date:  2011-06-17     Completed Date:  2011-11-01     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  101490775     Medline TA:  Am J Rhinol Allergy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  89-93     Citation Subset:  IM    
Affiliation:
Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg, Sweden. cecilia.lasser@gu.se
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / biosynthesis
Biological Markers / metabolism*
Bodily Secretions / metabolism*
Calnexin / biosynthesis
Cell Communication
Cell Separation
DNA-Binding Proteins / biosynthesis
Endosomal Sorting Complexes Required for Transport / biosynthesis
Exosomes / genetics,  metabolism*,  ultrastructure
Flow Cytometry
Humans
Microscopy, Electron
Nose / metabolism*,  pathology
RNA / genetics,  metabolism*
Transcription Factors / biosynthesis
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Biological Markers; 0/DNA-Binding Proteins; 0/Endosomal Sorting Complexes Required for Transport; 0/Transcription Factors; 0/Tsg101 protein; 139873-08-8/Calnexin; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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