| RNA-seq analysis of sulfur-deprived Chlamydomonas cells reveals aspects of acclimation critical for cell survival. | |
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MedLine Citation:
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PMID: 20587772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells. This mutant is null for the regulatory Ser-Thr kinase SNRK2.1, which is required for acclimation of the alga to sulfur deprivation. The transcriptome analyses used microarray hybridization and RNA-seq technology. Quantitative RT-PCR evaluation of the results obtained by these techniques showed that RNA-seq reports a larger dynamic range of expression levels than do microarray hybridizations. Transcripts responsive to sulfur deprivation included those encoding proteins involved in sulfur acquisition and assimilation, synthesis of sulfur-containing metabolites, Cys degradation, and sulfur recycling. Furthermore, we noted potential modifications of cellular structures during sulfur deprivation, including the cell wall and complexes associated with the photosynthetic apparatus. Moreover, the data suggest that sulfur-deprived cells accumulate proteins with fewer sulfur-containing amino acids. Most of the sulfur deprivation responses are controlled by the SNRK2.1 protein kinase. The snrk2.1 mutant exhibits a set of unique responses during both sulfur-replete and sulfur-depleted conditions that are not observed in wild-type cells; the inability of this mutant to acclimate to S deprivation probably leads to elevated levels of singlet oxygen and severe oxidative stress, which ultimately causes cell death. The transcriptome results for wild-type and mutant cells strongly suggest the occurrence of massive changes in cellular physiology and metabolism as cells become depleted for sulfur and reveal aspects of acclimation that are likely critical for cell survival. |
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Authors:
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David González-Ballester; David Casero; Shawn Cokus; Matteo Pellegrini; Sabeeha S Merchant; Arthur R Grossman |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-06-29 |
Journal Detail:
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Title: The Plant cell Volume: 22 ISSN: 1532-298X ISO Abbreviation: Plant Cell Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-29 Completed Date: 2010-10-18 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2058-84 Citation Subset: IM |
Affiliation:
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Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305, USA. q62gobad@uco.es |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE17970 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Chlamydomonas reinhardtii / genetics*, metabolism Gene Expression Profiling* Molecular Sequence Data Oligonucleotide Array Sequence Analysis / methods RNA, Algal / genetics*, metabolism RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Analysis, RNA / methods* Sulfur / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Algal; 0/RNA, Messenger; 7704-34-9/Sulfur |
| Comments/Corrections | |
Erratum In:
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Plant Cell. 2011 Apr;23(4):1679-81 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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