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RNA Polymerase V targets transcriptional silencing components to promoters of protein-coding genes.
MedLine Citation:
PMID:  23013441     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Transcriptional gene silencing controls transposons and other repetitive elements through RNA-directed DNA methylation (RdDM) and heterochromatin formation. A key component of the Arabidopsis RdDM pathway is ARGONAUTE4 (AGO4), which associates with siRNAs to mediate DNA methylation. Here, we show that AGO4 preferentially targets transposable elements embedded within promoters of protein-coding genes. We find that this pattern of AGO4 binding cannot be simply explained by the sequences of AGO4-bound siRNAs, but instead AGO4 binding to specific gene promoters is also mediated by long non-coding RNAs (lncRNAs) produced by RNA Polymerase V. lncRNA-mediated AGO4 binding to gene promoters directs asymmetric DNA methylation to these genomic regions and is involved in regulating the expression of targeted genes. Finally, AGO4-binding overlaps sites of DNA methylation affected by biotic stress response. Based on these findings, we propose that targets of AGO4-directed RdDM are regulatory units responsible for controlling gene expression under specific environmental conditions. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Authors:
Qi Zheng; M Jordan Rowley; Gudrun Böhmdorfer; Davinder Sandhu; Brian D Gregory; Andrzej T Wierzbicki
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Affiliation:
University of Pennsylvania, Department of Biology, Penn Genomes Frontier Institute, Philadelphia, PA, USA.
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