Document Detail


A RING-Type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE1 in Arabidopsis.
MedLine Citation:
PMID:  23347376     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Suppression of the expression of DEFECTIVE IN ANTHER DEHISCENCE1- (DAD1-) Activating Factor (DAF), a gene that encodes a putative RING-finger E3 ligase protein, causes non-dehiscence of the anthers, alters pollen development and causes sterility in 35S:DAF RNAi/antisense Arabidopsis plants. This mutant phenotype correlates with the suppression of DAF but not with the expression of the two most closely related genes, DAFL1/2. The expression of DAD1 was significantly reduced in 35S:DAF RNAi/antisense plants and 35S:DAF could not rescue the dad1 mutant, indicating that DAF acts upstream of DAD1 in JA biosynthesis. This assumption is supported by that 35S:DAF RNAi/antisense showed a similar cellular basis for anther dehiscence as that found in dad1 mutants and that the external application of JA rescued the anther non-dehiscence and pollen defects in the 35S:DAF antisense flowers. We further demonstrate that DAF is an E3 ubiquitin ligase and that its activity is abolished by C132S and H137Y mutations in its RING motif. Furthermore, ectopic expression of the dominant-negative C132S or H137Y mutation causes similar indehiscence of anthers and reduction in DAD1 expression in the transgenic Arabidopsis. This result not only confirms that DAF controls anther dehiscence by positively regulating the expression of DAD1 in the JA biosynthesis pathway but also supports the notion that DAF functions as an E3 ubiquitin ligase and that the conserved RING finger region is required for its activity. © 2013 The Authors. The Plant Journal © 2013 Blackwell Publishing Ltd.
Authors:
Yan-Jhu Peng; Ching-Fang Shih; Jun-Yi Yang; Choon-Meng Tan; Wei-Han Hsu; Yu-Pei Huang; Pei-Chun Liao; Chang-Hsien Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. The Plant Journal © 2013 Blackwell Publishing Ltd.
Affiliation:
Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan, 40227 ROC.
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