Document Detail

RIM-binding protein, a central part of the active zone, is essential for neurotransmitter release.
MedLine Citation:
PMID:  22174254     Owner:  NLM     Status:  In-Process    
The molecular machinery mediating the fusion of synaptic vesicles (SVs) at presynaptic active zone (AZ) membranes has been studied in detail, and several essential components have been identified. AZ-associated protein scaffolds are viewed as only modulatory for transmission. We discovered that Drosophila Rab3-interacting molecule (RIM)-binding protein (DRBP) is essential not only for the integrity of the AZ scaffold but also for exocytotic neurotransmitter release. Two-color stimulated emission depletion microscopy showed that DRBP surrounds the central Ca(2+) channel field. In drbp mutants, Ca(2+) channel clustering and Ca(2+) influx were impaired, and synaptic release probability was drastically reduced. Our data identify RBP family proteins as prime effectors of the AZ scaffold that are essential for the coupling of SVs, Ca(2+) channels, and the SV fusion machinery.
Karen S Y Liu; Matthias Siebert; Sara Mertel; Elena Knoche; Stephanie Wegener; Carolin Wichmann; Tanja Matkovic; Karzan Muhammad; Harald Depner; Christoph Mettke; Johanna Bückers; Stefan W Hell; Martin Müller; Graeme W Davis; Dietmar Schmitz; Stephan J Sigrist
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  334     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1565-9     Citation Subset:  IM    
Department of Genetics, Institute for Biology, Free University Berlin, 14195 Berlin, Germany.
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