Document Detail


RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids.
MedLine Citation:
PMID:  23064150     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill-defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria to the cytoplasm of infected cells. We found that Listeria released nucleic acids and that such secreted bacterial RNA/DNA was recognized by the cytosolic sensors RIG-I, MDA5 and STING thereby triggering interferon β production. Secreted Listeria nucleic acids also caused RIG-I-dependent IL-1β-production and inflammasome activation. The signalling molecule CARD9 contributed to IL-1β production in response to secreted nucleic acids. In conclusion, cytosolic recognition of secreted bacterial nucleic acids by RIG-I provides a mechanistic explanation for efficient induction of immunity by live bacteria.
Authors:
Zeinab Abdullah; Martin Schlee; Susanne Roth; Mobarak Abu Mraheil; Winfried Barchet; Jan Böttcher; Torsten Hain; Sergej Geiger; Yoshihiro Hayakawa; Jörg H Fritz; Filiz Civril; Karl-Peter Hopfner; Christian Kurts; Jürgen Ruland; Gunther Hartmann; Trinad Chakraborty; Percy A Knolle
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-12
Journal Detail:
Title:  The EMBO journal     Volume:  31     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-01-10     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  4153-64     Citation Subset:  IM    
Affiliation:
Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics,  metabolism
Animals
Blotting, Western
Cells, Cultured
Cytoplasm / immunology,  metabolism*,  microbiology
DEAD-box RNA Helicases / genetics,  metabolism,  physiology*
DNA, Bacterial / genetics,  immunology*
Flow Cytometry
Fluorescent Antibody Technique
Immunity, Cellular / immunology*
Inflammation / immunology*,  microbiology
Listeria monocytogenes / genetics,  immunology*
Listeriosis / genetics,  immunology*,  microbiology
Macrophages / immunology,  microbiology
Membrane Proteins / genetics,  metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
RNA, Bacterial / genetics,  immunology*
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Card9 protein, mouse; 0/DNA, Bacterial; 0/MPYS protein, mouse; 0/Membrane Proteins; 0/RNA, Bacterial; 0/RNA, Messenger; EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/Ddx58 protein, mouse; EC 3.6.1.-/Ifih1 protein, mouse
Comments/Corrections

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