Document Detail


RIFLE: rapid identification of microorganisms by fragment length evaluation.
MedLine Citation:
PMID:  9322027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biological macromolecules represent a valuable source of information for the identification and phylogenetic classification of microorganisms. One of the most commonly used macromolecules for this task is the 16S rDNA. The WWW-based RIFLE system presented here supports large-scale identification tasks by comparing 16S rDNA restriction patterns to a database of restriction patterns derived from sequence databases. Computing efficiency and robustness against experimental errors are gained by employing a new distance measure for restriction patterns, the fragment length distance. Results from the application of the system to the identification of uncultured microorganisms associated with the seagrass halophila stipulacea show the reliability of the method.
Authors:
H Hermjakob; R Giegerich; W Arnold
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Proceedings / ... International Conference on Intelligent Systems for Molecular Biology ; ISMB. International Conference on Intelligent Systems for Molecular Biology     Volume:  5     ISSN:  1553-0833     ISO Abbreviation:  Proc Int Conf Intell Syst Mol Biol     Publication Date:  1997  
Date Detail:
Created Date:  1997-12-11     Completed Date:  1997-12-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9509125     Medline TA:  Proc Int Conf Intell Syst Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  131-9     Citation Subset:  IM    
Affiliation:
GBF Braunschweig. hhe@gbf-braunschweig.de
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Bacteria / classification,  genetics,  isolation & purification
Computer Communication Networks
DNA Restriction Enzymes*
DNA, Ribosomal / genetics*
Databases, Factual*
Evaluation Studies as Topic
Poaceae / microbiology
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics
Reproducibility of Results
Restriction Mapping
Software*
Chemical
Reg. No./Substance:
0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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