Document Detail

RGD-Peptide Functionalized Graphene Biomimetic Live-Cell Sensor for Real Time Detection of Nitric Oxide Molecule.
MedLine Citation:
PMID:  22793649     Owner:  NLM     Status:  Publisher    
It is always challenging to construct a smart functional nanostructure with specific physico-chemical properties to real time detect bio-interesting molecules released from live-cells. We report here a new approach to build a free-standing biomimetic sensor by covalently bonding RGD-peptide on surface of pyrenebutyric acid functionalized graphene film. The resulted graphene biofilm sensor comprises a well-packed layered nanostructure, in which the RGD-peptide component provides desired biomimetic properties for superior human cell attachment and growth on the film surface to allow real time detecting nitric oxide, an important signal yet short life molecule released from the attached human endothelial cells under drug stimulations. The film sensor exhibits good flexibility and stability by retaining its original response after 45 bending/relaxing cycles, high reproducibility from its almost unchanged current responses after 15 repeated measurements, while possessing high sensitivity, good selectivity against interferences often existing in biological systems, demonstrating real time quantitative detection capability towards nitric oxide molecule released from living cells. This study not only demonstrates a facial approach to fabricate a smart nanostructured graphene-based functional biofilm, but also provides a powerful and reliable platform to real time study bio-interesting molecules released from living cells, thus rendering potential broad applications in neuroscience, screening drug therapy effect and live-cell assays.
Chun Xian Guo; Shu Rui Ng; Si Yun Khoo; Xinting Zheng; Peng Chen; Chang Ming Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-15
Journal Detail:
Title:  ACS nano     Volume:  -     ISSN:  1936-086X     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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