Document Detail

RET finger protein expression in invasive breast carcinoma: relationship between RFP and ErbB2 expression.
MedLine Citation:
PMID:  19232840     Owner:  NLM     Status:  MEDLINE    
RET finger protein (RFP), which belongs to the large B-box RING finger protein family, has been reported to be expressed in breast carcinoma cell lines. We conducted this study to evaluate the expression level of RFP in breast carcinomas. Tissue microarrays were constructed from 133 cases of invasive breast carcinoma. Sections obtained from tissue arrays and whole sections from 10 non-neoplastic breast tissues were stained for ER, PR, ErbB2, and RFP using immunohistochemistry, and ErbB2 gene status was evaluated by FISH. The correlation between various immunohistochemical markers was determined. We found nuclear RFP expression in 41.4% of invasive carcinomas and in none of the non-neoplastic breast tissues. We also stained whole sections of 29 cases of invasive breast carcinoma, which included the TMA study, and 10 cases of ductal carcinoma in situ (DCIS) by RFP. We observed that four (40%) of the DCIS cases were positive with RFP. In statistical analysis, there was a significant, positive association between RFP and ErbB2 status at the protein level (p=0.002) and the gene level (p=0.049). There was no statistically significant association between the expression of RFP and tumor size, LN status, ER, PR, and grade (p>0.05). However, we found a significant association between age and RFP expression. RFP expression was stronger in patients 50 years or older (p=0.048). In our study, the expression of RFP correlated strongly with ErbB2 status. Our study is the first in the literature to show expression of RFP in patients with breast carcinoma. However, the possible role of RFP in breast carcinogenesis needs to be investigated in more detailed clinical and experimental studies.
Gaye Guler Tezel; Aysegul Uner; Isil Yildiz; Gulnur Guler; Masahide Takahashi
Publication Detail:
Type:  Journal Article     Date:  2009-02-20
Journal Detail:
Title:  Pathology, research and practice     Volume:  205     ISSN:  1618-0631     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  2009  
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-08-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  403-8     Citation Subset:  IM    
Hacettepe University, Faculty of Medicine, Department of Pathology, Sihhiye 06100, Ankara, Turkey.
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MeSH Terms
Age Factors
Aged, 80 and over
Breast Neoplasms / genetics,  metabolism*,  pathology
Carcinoma in Situ / genetics,  metabolism,  pathology
Carcinoma, Ductal, Breast / genetics,  metabolism*,  pathology
DNA-Binding Proteins / biosynthesis*
Gene Expression
In Situ Hybridization, Fluorescence
Middle Aged
Neoplasm Staging
Nuclear Proteins / biosynthesis*
Receptor, erbB-2 / biosynthesis*
Receptors, Estrogen / biosynthesis
Receptors, Progesterone / biosynthesis
Tissue Array Analysis
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Receptors, Estrogen; 0/Receptors, Progesterone; 0/TRIM27 protein, human; 0/Tumor Markers, Biological; EC protein, human; EC, erbB-2

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