Document Detail

MedLine Citation:
PMID:  23013444     Owner:  NLM     Status:  Publisher    
Soluble and membrane-bound peroxidases (POD) were extracted from red cabbage using Triton X-114. Optimum activity was obtained at pH 4.0 for both enzymes and both were inactivated by the sodium dodecil sulphate (SDS). The KM and Vm values for H2O2 were found to be 0.98 mM and 8.1 M/min, respectively, for soluble POD; and 0.82 mM and 6.1 M/min, respectively, for membrane-bound POD. When the ABTS concentration was increased, maintaining the concentration of H2O2 steady, the activity was inhibited at the highest ABTS concentrations in soluble POD. Ascorbic acid was found to be the most active modulator of POD activity. The effect of cyclodextrins was also studied and the complexation constant between ABTS and HP--CDs was calculated (Kc = 312 M-1). Membrane-bound POD is more thermostable than soluble POD, losing > 90% of relative activity after 5 min of incubation at 76.6 ºC and 30.2 ºC, respectively.
Isabel Mi Fortea; José Antonio Ja Pellicer; Ana A Serrano; Santiago S López-Miranda; Carmen C Lucas-Abellán; Estrella Nuñez-Delicado
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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