| RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model. | |
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MedLine Citation:
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PMID: 18162370 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interstitial cystitis (IC) is a chronic inflammatory condition of the urinary bladder with a strong autoimmune component. Currently, the major challenge in IC treatment is the development of effective therapies. RDP58 is a novel d-amino acid decapeptide with potent immunosuppressive activity. In this study, we investigated whether RDP58 was effective as an intravesical agent for treating bladder autoimmune inflammation in a transgenic mouse model (URO-OVA mice). URO-OVA mice were adoptively transferred with syngeneic activated splenocytes of OT-I mice transgenic for the OVA-specific CD8(+) TCR for cystitis induction and treated intravesically with RDP58 at days 0 and 3. Compared with controls, the RDP58-treated bladders showed markedly reduced histopathology and expressions of mRNAs and proteins of TNF-alpha, NGF and substance P. To determine whether the inhibition of bladder inflammation by RDP58 was due to the interference with effector T cells, we treated the cells with RDP58 in vitro. Cells treated with RDP58 showed reduced production of TNF-alpha and IFN-gamma as well as apoptotic death. Collectively, these results indicate that RDP58 is effective for treating T cell-mediated experimental autoimmune cystitis and may serve as a useful intravesical agent for the treatment of autoimmune-associated bladder inflammation such as IC. |
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Authors:
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Wujiang Liu; Barry R Deyoung; Xiaohong Chen; David P Evanoff; Yi Luo |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-12-26 |
Journal Detail:
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Title: Journal of autoimmunity Volume: 30 ISSN: 0896-8411 ISO Abbreviation: J. Autoimmun. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-04-28 Completed Date: 2008-07-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8812164 Medline TA: J Autoimmun Country: England |
Other Details:
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Languages: eng Pagination: 257-65 Citation Subset: IM |
Affiliation:
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Department of Urology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, 3202 MERF, 375 Newton Road, IA 52242-1087, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoimmune Diseases / drug therapy*, immunology, pathology CD8-Positive T-Lymphocytes / drug effects*, immunology Cystitis / drug therapy*, immunology, pathology Cytokines / biosynthesis, drug effects Disease Models, Animal Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Immunosuppressive Agents / administration & dosage* Injections, Intralesional Mice Mice, Transgenic Peptides / administration & dosage* Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK066079/DK/NIDDK NIH HHS; R01 DK066079-03/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Immunosuppressive Agents; 0/Peptides; 151232-75-6/allotrap |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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