Document Detail

RASSF1A promoter methylation and Kras2 mutations in non small cell lung cancer.
MedLine Citation:
PMID:  14511407     Owner:  NLM     Status:  MEDLINE    
In the present studies, we investigated the correlation between RASSF1A promoter methylation status and Kras2 mutations in 65 primary non small cell lung cancer (NSCLC) including 33 adenocarcinomas, 12 large cell carcinomas, and 20 squamous cell carcinomas. Mutational analysis of Kras2 showed: 30% (10 of 33) of adenocarcinomas, 25% (3 of 12) of large cell carcinomas, and only 5% (1 of 20) of squamous cell carcinomas contained activated Kras2 mutation at codon 12 or 13. RASSF1A promoter region CpG island methylation was detected in adenocarcinomas (55%), large cell carcinomas (25%), and squamous cell carcinomas (25%). Interestingly, combined RASSF1A methylation and Kras2 mutation data show that only - 7% adenocarcinomas/large cell carcinomas exhibited both KRASSF1A promoter methylation and Kras2 mutation, whereas 24% adenocarcinomas, 50% large cell carcinomas, and 70% squamous cell carcinomas showed neither Kras2 mutation nor RASSF1A promoter methylation. These results showed that the majority of the primary NSCLCs with Kras2 mutations lack RASSF1A inactivation, and both RASSF1A inactivation and Kras2 mutation events occur frequently in adenocarcinomas and large cell carcinomas. Our results indicate a trend of inverse relationship between Kras2 activation and RASSF1A promoter methylation in the majority of human lung adenocarcinomas and large cell carcinomas.
Jie Li; Zhongqiu Zhang; Zunyan Dai; Anthony P Popkie; Christoph Plass; Carl Morrison; Yian Wang; Ming You
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neoplasia (New York, N.Y.)     Volume:  5     ISSN:  1522-8002     ISO Abbreviation:  Neoplasia     Publication Date:    2003 Jul-Aug
Date Detail:
Created Date:  2003-09-26     Completed Date:  2004-04-30     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  100886622     Medline TA:  Neoplasia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  362-6     Citation Subset:  IM    
Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
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MeSH Terms
Adenocarcinoma / genetics
Carcinoma, Large Cell / genetics
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Squamous Cell / genetics
Cell Line, Tumor
Colonic Neoplasms / genetics
DNA / metabolism
DNA Methylation*
Lung Neoplasms / genetics*
Polymerase Chain Reaction
Promoter Regions, Genetic*
Proto-Oncogene Proteins / genetics*
Sequence Analysis, DNA
Signal Transduction
Tumor Suppressor Proteins / genetics*
ras Proteins
Grant Support
Reg. No./Substance:
0/KRAS protein, human; 0/Proto-Oncogene Proteins; 0/RASSF1 protein, human; 0/Tumor Suppressor Proteins; 9007-49-2/DNA; EC Proteins

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