Document Detail


RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress.
MedLine Citation:
PMID:  21042774     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Activation of the receptor for advanced glycation endproducts (RAGE) by its ligands leads to cellular damage contributing to diabetic complications. It is not clearly known whether RAGE ligands influence pancreatic β-cells. In this study, we investigated the expression of RAGE in islet cells and the effect of RAGE ligands, S100b and HMG-1, on islet cells. RAGE was expressed in INS-1 cells and isolated rat and human islets at mRNA and protein levels. RAGE and its ligand, S100b, were detected on islet cells in 28-week-old diabetic OLETF rats. Both S100b and HMG-1 induced apoptotic cell death of INS-1 and islet cells. This INS-1 cell apoptosis was accompanied by increased intracellular oxidative stress and inhibited by antioxidants or a NADPH oxidase inhibitor. Our results showing S100b/RAGE expression on islets of diabetic rat model and RAGE ligands-induced islet cell apoptosis via NADPH oxidase-mediated ROS generation suggest that RAGE ligands-RAGE interaction may contribute not only to the development of diabetic complications but also to the progressive β-cell loss in type 2 diabetes by inducing oxidative stress.
Authors:
Byung-Wan Lee; Hee Young Chae; Soo Jin Kwon; So Youn Park; Jahei Ihm; Sung-Hee Ihm
Related Documents :
7518824 - Heterogeneity and contact-dependent regulation of amylase release by individual acinar ...
6750614 - Expression of receptors for tetanus toxin and monoclonal antibody a2b5 by pancreatic is...
2153524 - Different expression of protein kinase c isozymes in pancreatic islet cells.
8216384 - The effects of long-term administration of 3-thia fatty acid, a peroxisome proliferator...
9639394 - Changes in glutathione redox cycling and oxidative stress response in the malignant pro...
18624324 - Microencapsulation of recombinant saccharomyces cerevisiae cells with invertase activit...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  26     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  813-8     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Hallym University College of Medicine, 896 Pyungchon-dong, Kyonggi-do, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and...
Next Document:  MicroRNA-21 promotes the cell proliferation, invasion and migration abilities in ovarian epithelial ...