| RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40. | |
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MedLine Citation:
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PMID: 16888649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ubiquitination of proliferating-cell nuclear antigen (PCNA) at K164 by RAD6/RAD18 has a key role in DNA damage tolerance in yeast. Here, we report on the first genetic study of this modification in a vertebrate cell. As in yeast, mutation of K164 of PCNA to arginine in the avian cell line DT40 results in sensitivity to DNA damage but, by contrast, the DT40 pcnaK164R mutant is more sensitive than the rad18 mutant. Consistent with this, we show the presence of residual ubiquitination of PCNA at K164 in the absence of functional RAD18, suggesting the presence of an alternate PCNA ubiquitinating enzyme in DT40. Furthermore, RAD18 and PCNA K164 have non-overlapping roles in the suppression of sister chromatid exchange in DT40, showing that RAD18 has other functions that do not involve the ubiquitination of PCNA. |
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Authors:
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Laura J Simpson; Anna-Laura Ross; Dávid Szüts; Cherry A Alviani; Vibe H Oestergaard; Ketan J Patel; Julian E Sale |
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Publication Detail:
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Type: Journal Article Date: 2006-08-04 |
Journal Detail:
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Title: EMBO reports Volume: 7 ISSN: 1469-221X ISO Abbreviation: EMBO Rep. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-05 Completed Date: 2006-10-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100963049 Medline TA: EMBO Rep Country: England |
Other Details:
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Languages: eng Pagination: 927-32 Citation Subset: IM |
Affiliation:
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Medical Research Council Laboratory of Molecular Biology, Division of Protein & Nucleic Acid Chemistry, Hills Road, Cambridge CB2 2QH, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Proliferation Chickens DNA Damage* DNA Replication* Mutation Proliferating Cell Nuclear Antigen / genetics*, metabolism Protein Processing, Post-Translational Sister Chromatid Exchange Transfection Ubiquitin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Proliferating Cell Nuclear Antigen; 0/Ubiquitin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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