Document Detail

RACK1 functions in rice innate immunity by interacting with the Rac1 immune complex.
MedLine Citation:
PMID:  18723578     Owner:  NLM     Status:  MEDLINE    
A small GTPase, Rac1, plays a key role in rice (Oryza sativa) innate immunity as part of a complex of regulatory proteins. Here, we used affinity column chromatography to identify rice RACK1 (for Receptor for Activated C-Kinase 1) as an interactor with Rac1. RACK1 functions in various mammalian signaling pathways and is involved in hormone signaling and development in plants. Rice contains two RACK1 genes, RACK1A and RACK1B, and the RACK1A protein interacts with the GTP form of Rac1. Rac1 positively regulates RACK1A at both the transcriptional and posttranscriptional levels. RACK1A transcription was also induced by a fungal elicitor and by abscisic acid, jasmonate, and auxin. Analysis of transgenic rice plants and cell cultures indicates that RACK1A plays a role in the production of reactive oxygen species (ROS) and in resistance against rice blast infection. Overexpression of RACK1A enhances ROS production in rice seedlings. RACK1A was shown to interact with the N terminus of NADPH oxidase, RAR1, and SGT1, key regulators of plant disease resistance. These results suggest that RACK1A functions in rice innate immunity by interacting with multiple proteins in the Rac1 immune complex.
Ayako Nakashima; Letian Chen; Nguyen Phuong Thao; Masayuki Fujiwara; Hann Ling Wong; Masayoshi Kuwano; Kenji Umemura; Ken Shirasu; Tsutomu Kawasaki; Ko Shimamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-22
Journal Detail:
Title:  The Plant cell     Volume:  20     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-10-01     Completed Date:  2009-01-16     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2265-79     Citation Subset:  IM    
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AAF18438;  AB029508;  AB029509;  AB029510;  AK058414;  AK061102;  AK067504;  AK100842;  AK111881;  AK121567;  AY325266;  D38170;  D38231
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MeSH Terms
Abscisic Acid / pharmacology
Chromatography, Affinity
Cyclopentanes / pharmacology
Gene Expression Regulation, Plant / drug effects
Host-Pathogen Interactions
Immunity, Innate / genetics
Indoleacetic Acids / pharmacology
Magnaporthe / physiology
Molecular Sequence Data
Oryza sativa / genetics,  metabolism*,  microbiology
Oxylipins / pharmacology
Plant Diseases / genetics,  microbiology
Plant Proteins / genetics,  metabolism*
Plants, Genetically Modified / genetics,  metabolism*,  microbiology
Protein Binding / drug effects
Reactive Oxygen Species / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tandem Mass Spectrometry
Two-Hybrid System Techniques
Reg. No./Substance:
0/Cyclopentanes; 0/Indoleacetic Acids; 0/Oxylipins; 0/Plant Proteins; 0/Reactive Oxygen Species; 21293-29-8/Abscisic Acid; 6RI5N05OWW/jasmonic acid

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