Document Detail


RACK1, a PKC targeting protein, is exclusively localized to basal airway epithelial cells.
MedLine Citation:
PMID:  17875659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The novel isoform of protein kinase C (PKC), PKCepsilon, is an important regulator of ciliated cell function in airway epithelial cells, including cilia motility and detachment of ciliated cells after environmental insult. However, the mechanism of PKCepsilon signaling in the airways and the potential role of the PKCepsilon-interacting protein, receptor for activated C kinase 1 (RACK1), has not been widely explored. We used immunohistochemistry and Western blot analysis to show that RACK1 is localized exclusively to basal, non-ciliated (and non-goblet) bovine and human bronchial epithelial cells. Our immunohistochemistry experiments used the basal body marker pericentrin, a marker for cilia, beta-tubulin, and an airway goblet cell marker, MUC5AC, to confirm that RACK1 was excluded from differentiated airway cell subtypes and is only expressed in the basal cells. These results suggest that PKCepsilon signaling in the basal airway cell may involve RACK1; however, PKCepsilon regulation in ciliated cells uses RACK1-independent pathways.
Authors:
Rebecca E Slager; Jane M Devasure; Jaqueline A Pavlik; Joseph H Sisson; Todd A Wyatt
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Publication Detail:
Type:  Journal Article     Date:  2007-09-17
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  56     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-18     Completed Date:  2008-01-22     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7-14     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Pulmonary and Critical Care Medicine Section, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198-5300, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bronchi / cytology,  metabolism*
Cattle
Cells, Cultured
Cilia / metabolism
Epithelial Cells / metabolism*
GTP-Binding Proteins / metabolism*
Humans
Immunohistochemistry
Neoplasm Proteins / metabolism*
Protein Kinase C-epsilon / metabolism*
Receptors, Cell Surface / metabolism*
Respiratory Mucosa / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/GNB2L1 protein, human; 0/Neoplasm Proteins; 0/Receptors, Cell Surface; EC 2.7.11.13/Protein Kinase C-epsilon; EC 3.6.1.-/GTP-Binding Proteins
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