| RAB-5 controls the cortical organization and dynamics of PAR proteins to maintain C. elegans early embryonic polarity. | |
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MedLine Citation:
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PMID: 22545101 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In all organisms, cell polarity is fundamental for most aspects of cell physiology. In many species and cell types, it is controlled by the evolutionarily conserved PAR-3, PAR-6 and aPKC proteins, which are asymmetrically localized at the cell cortex where they define specific domains. While PAR proteins define the antero-posterior axis of the early C. elegans embryo, the mechanism controlling their asymmetric localization is not fully understood. Here we studied the role of endocytic regulators in embryonic polarization and asymmetric division. We found that depleting the early endosome regulator RAB-5 results in polarity-related phenotypes in the early embryo. Using Total Internal Reflection Fluorescence (TIRF) microscopy, we observed that PAR-6 is localized at the cell cortex in highly dynamic puncta and depleting RAB-5 decreased PAR-6 cortical dynamics during the polarity maintenance phase. Depletion of RAB-5 also increased PAR-6 association with clathrin heavy chain (CHC-1) and this increase depended on the presence of the GTPase dynamin, an upstream regulator of endocytosis. Interestingly, further analysis indicated that loss of RAB-5 leads to a disorganization of the actin cytoskeleton and that this occurs independently of dynamin activity. Our results indicate that RAB-5 promotes C. elegans embryonic polarity in both dynamin-dependent and -independent manners, by controlling PAR-6 localization and cortical dynamics through the regulation of its association with the cell cortex and the organization of the actin cytoskeleton. |
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Authors:
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Vincent Hyenne; Thierry Tremblay-Boudreault; Ramraj Velmurugan; Barth D Grant; Dinah Loerke; Jean-Claude Labbé |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-04-24 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-04-30 Completed Date: 2012-08-27 Revised Date: 2013-03-11 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e35286 Citation Subset: IM |
Affiliation:
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Institute of Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada. worm.machine@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actomyosin
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metabolism Animals Caenorhabditis elegans / embryology*, genetics, metabolism Caenorhabditis elegans Proteins / analysis, genetics, metabolism* Cell Polarity* Dynamins / metabolism Embryo, Nonmammalian / cytology*, embryology, metabolism Gene Expression Regulation, Developmental Phenotype Vesicular Transport Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM067237/GM/NIGMS NIH HHS; R01 GM067237/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/Rab-5 protein, C elegans; 0/Vesicular Transport Proteins; 0/par-6 protein, C elegans; 9013-26-7/Actomyosin; EC 3.6.5.5/Dynamins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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