Document Detail


RAB-5 controls the cortical organization and dynamics of PAR proteins to maintain C. elegans early embryonic polarity.
MedLine Citation:
PMID:  22545101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In all organisms, cell polarity is fundamental for most aspects of cell physiology. In many species and cell types, it is controlled by the evolutionarily conserved PAR-3, PAR-6 and aPKC proteins, which are asymmetrically localized at the cell cortex where they define specific domains. While PAR proteins define the antero-posterior axis of the early C. elegans embryo, the mechanism controlling their asymmetric localization is not fully understood. Here we studied the role of endocytic regulators in embryonic polarization and asymmetric division. We found that depleting the early endosome regulator RAB-5 results in polarity-related phenotypes in the early embryo. Using Total Internal Reflection Fluorescence (TIRF) microscopy, we observed that PAR-6 is localized at the cell cortex in highly dynamic puncta and depleting RAB-5 decreased PAR-6 cortical dynamics during the polarity maintenance phase. Depletion of RAB-5 also increased PAR-6 association with clathrin heavy chain (CHC-1) and this increase depended on the presence of the GTPase dynamin, an upstream regulator of endocytosis. Interestingly, further analysis indicated that loss of RAB-5 leads to a disorganization of the actin cytoskeleton and that this occurs independently of dynamin activity. Our results indicate that RAB-5 promotes C. elegans embryonic polarity in both dynamin-dependent and -independent manners, by controlling PAR-6 localization and cortical dynamics through the regulation of its association with the cell cortex and the organization of the actin cytoskeleton.
Authors:
Vincent Hyenne; Thierry Tremblay-Boudreault; Ramraj Velmurugan; Barth D Grant; Dinah Loerke; Jean-Claude Labbé
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-04-24
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-30     Completed Date:  2012-08-27     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e35286     Citation Subset:  IM    
Affiliation:
Institute of Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada. worm.machine@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Actomyosin / metabolism
Animals
Caenorhabditis elegans / embryology*,  genetics,  metabolism
Caenorhabditis elegans Proteins / analysis,  genetics,  metabolism*
Cell Polarity*
Dynamins / metabolism
Embryo, Nonmammalian / cytology*,  embryology,  metabolism
Gene Expression Regulation, Developmental
Phenotype
Vesicular Transport Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
GM067237/GM/NIGMS NIH HHS; R01 GM067237/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Rab-5 protein, C elegans; 0/Vesicular Transport Proteins; 0/par-6 protein, C elegans; 9013-26-7/Actomyosin; EC 3.6.5.5/Dynamins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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