| (R)-citramalate synthase in methanogenic archaea. | |
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MedLine Citation:
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PMID: 9864346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine. |
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Authors:
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D M Howell; H Xu; R H White |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 181 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-02-05 Completed Date: 1999-02-05 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 331-3 Citation Subset: IM; S |
Affiliation:
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Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases Acyltransferases Base Sequence Cloning, Molecular DNA Primers / genetics Escherichia coli / genetics Gene Expression Genes, Archaeal Kinetics Malates / metabolism* Methanococcus / enzymology*, genetics* Oxo-Acid-Lyases / genetics*, isolation & purification, metabolism* Recombinant Proteins / genetics, isolation & purification, metabolism Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Malates; 0/Recombinant Proteins; 2306-22-1/citramalate; EC 2.3.-/Acyltransferases; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.182/(R)-citramalate synthase; EC 4.1.3.-/Oxo-Acid-Lyases |
| Comments/Corrections | |
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