Document Detail


Quick and reliable assessment of chronological life span in yeast cell populations by flow cytometry.
MedLine Citation:
PMID:  21736893     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Yeast chronological life span (CLS) is defined as the capacity of stationary cultures to maintain viability over time, thus mimicking the situation of post-mitotic cells in multicellular organisms. Cellular viability is typically determined by using the colony formation unit assay (CFU). CFU counting is simple but laborious and does not accommodate large-scale experiments. Importantly, viability is determined on the basis of the cell's ability to divide and form a colony, probably not the ideal parameter when studying post-mitotic cellular ageing. This study describes the optimization and validation of a method based on the flow cytometric monitoring of propidium iodide (PI) uptake for assessing yeast cell death during CLS. The optimized protocol is quick, reliable, reproducible and can accommodate high-throughput studies. The method was validated by determining CLS of several strains used in yeast ageing research and by evaluating the effect of genetic disturbances known to extend or reduce yeast chronological life span.
Authors:
Alejandro Ocampo; Antoni Barrientos
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-28
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  -     ISSN:  1872-6216     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-7-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ireland Ltd.
Affiliation:
Department of Biochemistry & Molecular Biology, University of Miami Miller School of Medicine, Miami, FL, United States.
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