| Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method. | |
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MedLine Citation:
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PMID: 17401335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here, we describe a quick and easy version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method can be performed when only a few transformants are needed. The procedure can take less than an hour, depending on the duration of the heat shock. It can be used to transform yeast cells from various stages of growth and storage. Cells can be transformed from freshly grown cells as well as cells stored on a plate at room temperature or in a refrigerator. |
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Authors:
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R Daniel Gietz; Robert H Schiestl |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature protocols Volume: 2 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-02 Completed Date: 2007-06-29 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 35-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates DNA, Single-Stranded / genetics Genetic Techniques* Hot Temperature Polyethylene Glycols Saccharomyces cerevisiae / cytology, genetics* Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/DNA, Single-Stranded; 0/Polyethylene Glycols; 0/lithium acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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