| Quercetin suppresses NF-κB and MCP-1 expression in a high glucose-induced human mesangial cell proliferation model. | |
| | |
MedLine Citation:
|
PMID: 22469745 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Diabetic nephropathy (DN), which is characterized by mesangial cell proliferation, is a common complication observed in diabetic patients. The protective effects of quercetin for DN have been reported; however, the mechanism has yet to be determined. We aimed to identify the underlying mechanism for quercetin protection against DN. High glucose (HG)-induced human mesangial cell (HMC) proliferation, a feature of the early stages of diabetic nephropathy, was employed as an in vitro model. Cells were grown in normal glucose (5.6 mM), high glucose (30 mM) or high glucose with various concentrations of quercetin. Cell proliferation, cell cycle progression, and expression of NF-κB and MCP-1 were examined by MTT assay, DNA staining, immunocytochemistry and western blot analysis, respectively. HMCs cultured in high glucose had signficantly greater proliferation, accumulation in the G1 phase, upregulated NF-κB and MCP-1 expression. Quercetin treatment reversed the effects of high glucose in a dose-dependent manner. Cotreatment of quercetin with pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB activation, suggest that the effects of quercetin are partially mediated by NF-κB signaling. Quercetin partially suppresses the effects of high glucose in HMC cultures, which are mediated at least in part through the suppression of NF-κB. |
| | |
Authors:
|
Pin Chen; Qiyang Shi; Xiangjin Xu; Yanqiao Wang; Wenyu Chen; Huiling Wang |
Related Documents
:
|
7259065 - Prolactin and impotence in diabetes mellitus. 3155475 - Comparative investigations in 26 impotent and 26 nonimpotent diabetic patients. 18290855 - Efficacy of tadalafil once daily in men with diabetes mellitus and erectile dysfunction. 8485425 - Modelling the interactions between alcohol, crime and the criminal justice system. 8560405 - Influence of various heparin preparations on lipoproteins in hemodialysis patients: a m... 12441885 - Lipid-lowering therapy in people with type 2 diabetes. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-02 |
Journal Detail:
|
Title: International journal of molecular medicine Volume: - ISSN: 1791-244X ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Fuzhou General Hospital of Nanjing Military Command, Fuzhou, Fujian 350025, P.R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Radical Endoscopic Resection is Unsuitable for Most Synchronous, Multiple and Early Gastric Cancers.
Next Document: Association between polymorphisms of the DNA base excision repair genes MUTYH and hOGG1 and age-rela...