Document Detail


Quercetin protects hamster spermatogenic cells from oxidative damage induced by diethylstilboestrol.
MedLine Citation:
PMID:  20860625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quercetin has been reported to be an efficient antioxidant which protects chicken spermatogonial cells from oxidative damage through increasing intracellular antioxidants and decreasing lipid peroxidation. Exposure to diethylstilboestrol (DES) could cause reproductive damage in males, which is associated with oxidative stress. This study was conducted to investigate the protective effects of quercetin on DES-induced oxidative damage in cultured hamster spermatogenic cells. The cells were treated with different concentrations of DES, and their growth status was observed under inverted microscope. The viability of spermatogenic cells was detected by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT). The contents of superoxide dismutase (SOD) in supernatants and glutathione peroxidase (GSH-Px) in cells were detected with spectrophotography. The results showed that quercetin significantly inhibited the DES-induced damage on spermatogenic cells, with the exception of the low-dose group in which no significant difference was observed. The cell survival rate increased significantly in the middle- and high-dose groups. The contents of SOD and GSH-Px were significantly elevated after medication with quercetin (P < 0.01). It can be concluded that quercetin protects spermatogenic cells against DES-induced oxidative damage through increasing intracellular antioxidants and decreasing lipid peroxidation. Quercetin plays a very important role in ameliorating reproductive toxicity induced by environmental oestrogens.
Authors:
G Li; Aituan Ma; W Shi; Xiuhui Zhong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  42     ISSN:  1439-0272     ISO Abbreviation:  Andrologia     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-23     Completed Date:  2011-01-10     Revised Date:  2011-12-20    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  285-90     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Verlag GmbH.
Affiliation:
College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / pharmacology*
Cell Survival / drug effects
Cells, Cultured
Cricetinae
Cytoprotection
Diethylstilbestrol / toxicity*
Endocrine Disruptors / toxicity*
Glutathione Peroxidase / analysis
Lipid Peroxidation / drug effects
Male
Oxidative Stress / drug effects*
Quercetin / pharmacology*
Spermatogonia / drug effects
Spermatozoa / drug effects*
Superoxide Dismutase / analysis,  metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Endocrine Disruptors; 117-39-5/Quercetin; 56-53-1/Diethylstilbestrol; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

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