Document Detail


Quercetin inhibits human DLD-1 colon cancer cell growth and polyamine biosynthesis.
MedLine Citation:
PMID:  20944129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIM: Polyamines and ornithine decarboxylase are involved in cell growth and differentiation. The polyphenol quercetin may exert anti-tumour properties by influencing proliferation, differentiation, and apoptosis. The aim of the study was to investigate the effects of increasing concentrations of quercetin (from 0.1 to 100 μM) on polyamine biosynthesis, cell proliferation, and apoptosis in the DLD-1 cells. MATERIALS AND METHODS: Polyamine levels and ornithine decarboxylase activity were evaluated by HPLC and radiometric technique, respectively. The proliferative response was estimated by 3-(4,5 dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide (MTT) test and [(3)H]-thymidine incorporation in cell DNA. Apoptosis was investigated by DNA fragmentation. RESULTS: At concentrations ≥50 μM, quercetin significantly reduced ornithine decarboxylase activity, putrescine and spermidine levels compared to controls and cells treated with 0.1 μM concentration. Quercetin concentrations ≥70 μM caused a significant reduction in the conversion of MTT tetrazolium salt and [(3)H]-thymidine incorporation. The same concentrations were needed to induce the apoptosis. CONCLUSION: The present study demonstrates that quercetin can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis. Due to the extensive dietary consumption of polyphenols, such as quercetin, the biological activity of these compounds deserves further investigation.
Authors:
Michele Linsalata; Antonella Orlando; Caterina Messa; Maria Grazia Refolo; Francesco Russo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3501-7     Citation Subset:  IM    
Affiliation:
Laboratory of Experimental Biochemistry, Scientific Institute for Digestive Diseases, IRCCS Saverio de Bellis, I-70013 Castellana G, BA, Italy.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Biogenic Polyamines / biosynthesis*
Cell Line, Tumor
Cell Proliferation / drug effects*
Chromatography, High Pressure Liquid
Colonic Neoplasms / metabolism*
DNA Fragmentation
Dose-Response Relationship, Drug
Humans
Ornithine Decarboxylase / drug effects,  metabolism
Quercetin / pharmacology*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Biogenic Polyamines; 117-39-5/Quercetin; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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