Document Detail

Quenching of the Electrochemiluminescence of Tris(2,2'- bipyridine)ruthenium(II)/Tri-n-propylamine by Pristine Carbon Nanotube and Its Application to Quantitative Detection of DNA.
MedLine Citation:
PMID:  23311854     Owner:  NLM     Status:  Publisher    
In this study, we describe the quenching of electrochemiluminescence (ECL) of tris(2,2'-bipyridine)-ruthenium(II)(Ru(bpy)32+)/tri-n-propylamine(TPA) at pristine multiwall carbon nanotube (MWNT) modified glassy carbon (GC) electrode. Even that the faradic current of Ru(bpy)32+/TPA system and the oxidation of TPA obtained at pristine MWNT-modified GC electrode is enhanced compared with those at bare GC electrode, the intensity of ECL produced at MWNT electrode is smaller than that at GC electrode. For testing the possible reason of quenching, a comparison of ECL behavior of Ru(bpy)32+/TPA at pristine MWNT, acid-treated, heat-treated and polyethylene glycol (PEG)-wrapped MWNT-modified GC electrode is studied. The results demonstrate that the oxygen-containing groups at the surface of MWNT and the intrinsic electron properties of MWNT is considered to be the major reason for the suppression of ECL. The comparison also demonstrates that this quenching is related to the distance between MWNT and Ru(bpy)32+/TPA. Utilizing this essential quenching mechanism, a new signal-on DNA hybridization assay is proposed based on MWNT modified electrode, where single-stranded DNA (ssDNA) labeled with Ru(bpy)32+ derivatives probe (Ru-ssDNA) at the distal end is covalently attached onto the MWNT electrode. ECL signal is quenched where Ru-ssDNA is self-organized on the surface of MWNT electrode; however, the quenched ECL signal returns in case of the presence of complementary ssDNA. The developed approach for sequence-specific DNA detection has good selectivity, sensitivity and signal-to-background ratio. Therefore, the quenching of the ECL of Ru(bpy)32+/TPA system by the pristine MWNT can be an excellent platform for nucleic acid studies and molecular sensing.
Xiaofeng Tang; Dan Zhao; Jinchao He; Fengwang Li; Jiaxi Peng; Meining Zhang
Related Documents :
18952104 - The interrelationship of helicase and nuclease domains during dna translocation by the ...
20304994 - Reca4142 causes sos constitutive expression by loading onto reversed replication forks ...
20080104 - The single-stranded dna binding protein of sulfolobus solfataricus acts in the presynap...
23535144 - Ago/risc-mediated antiviral rna silencing in a plant in vitro system.
20350144 - Oxidative dna damage: biological significance and methods of analysis.
6173214 - Adeno-associated virus rna transcription in vivo.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The argument from potentiality in the embryo protection debate: finally "depotentialized"?
Next Document:  One-Pot Organocatalytic Enantioselective Domino Double-Michael Reaction and Pictet-Spengler-Lactamiz...