Document Detail


Quaternary structure of human fatty acid synthase by electron cryomicroscopy.
MedLine Citation:
PMID:  11756679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present the first three-dimensional reconstruction of human fatty acid synthase obtained by electron cryomicroscopy and single-particle image processing. The structure shows that the synthase is composed of two monomers, arranged in an antiparallel orientation, which is consistent with biochemical data. The monomers are connected to each other at their middle by a bridge of density, a site proposed to be the combination of the interdomain regions of the two monomers. Each monomer subunit appears to be subdivided into three structural domains. With this reconstruction of the synthase, we propose a location for the enzyme's two fatty acid synthesis sites.
Authors:
Jacob Brink; Steven J Ludtke; Chao-Yuh Yang; Zei-Wei Gu; Salih J Wakil; Wah Chiu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2001-12-26
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  99     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-09     Completed Date:  2002-04-15     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  138-43     Citation Subset:  IM    
Affiliation:
Verna and Marrs McLean Department of Biochemistry and Molecular Biology and National Center for Macromolecular Imaging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Cryoelectron Microscopy
Electrophoresis, Polyacrylamide Gel
Fatty Acid Synthetase Complex / chemistry*
Humans
Protein Structure, Quaternary
Protein Structure, Tertiary
Scattering, Radiation
Tumor Cells, Cultured
X-Rays
Grant Support
ID/Acronym/Agency:
GMS19091/GM/NIGMS NIH HHS; P41RR012109/RR/NCRR NIH HHS; P41RR02250/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
EC 6.-/Fatty Acid Synthetase Complex
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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