Document Detail

Quasi-real time quantification of uric acid in urine using boron doped diamond microelectrode with in-situ cleaning.
MedLine Citation:
PMID:  23126450     Owner:  NLM     Status:  Publisher    
We report herein an innovative electrochemical (EC) technique based on Boron doped diamond (BDD) microelectrodes which enables the fast determination of uric acid (UA) concentrations in urine. Based on fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared successfully using routine spectrophotometric diagnosis. The approach relies on the use of BDD's superior properties such as low background current, low adsorption of species, long term stability, and anti-fouling capabilities using electrochemical reactivation. Moreover, the article also describes an in-situ activation technique, where the electrodes were reactivated within human urine, thereby opening the way towards automatic quantification of UA with in-situ cleaning. The time taken to quantify UA concentration and cleaning remains below 0.5 seconds. Two analytic models were derived, based on different concentrations of ascorbic acid (AA) and uric acid, consisting of 2 second order calibration curves. Solving the second order equation enables the direct estimation of UA concentration, and values demonstrated good accuracy when compared with spectrophotometric measurements.
Raphael Kiran; Emmanuel Scorsone; Pascal Mailley; Philippe Bergonzo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-5
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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