| Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. | |
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MedLine Citation:
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PMID: 20171733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantum dots (QDs) have been used to study the effects of fluorescent probes for biomolecules and cell imaging. Adipose tissue-derived stem cells, which carry a relatively lower donor site morbidity, while yielding a large number of stem cells at harvest, were transduced with QDs using the octa-arginine peptide (R8) cell-penetrating peptide (CPP). The concentration ratio of QDs:R8 of 1 x 10(4) was optimal for delivery into ASCs. No cytotoxicity was observed in ASCs transduced with less than 16 nM of QDs655. In addition, >80% of the cells could be labeled within 1 h and the fluorescent intensity was maintained at least for 2 weeks. The ASCs transduced with QDs using R8 could be differentiated into both adipogenic and osteogenic cells, thus suggesting that the cells maintained their stem cell potency. The ASCs labeled with QDs using R8 were further transplanted subcutaneously into the backs of mice or into mice through the tail vein. The labeled ASCs could be imaged with good contrast using the Maestro in vivo imaging system. These data suggested that QD labeling using R8 could be utilized for the imaging of ASCs. |
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Authors:
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Hiroshi Yukawa; Yukimasa Kagami; Masaki Watanabe; Koichi Oishi; Yoshitaka Miyamoto; Yukihiro Okamoto; Manabu Tokeshi; Noritada Kaji; Hirofumi Noguchi; Kenji Ono; Makoto Sawada; Yoshinobu Baba; Nobuyuki Hamajima; Shuji Hayashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-19 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-06-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4094-103 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya University, Higashi-ku, Nagoya, Japan. hiroshiy@med.nagoya-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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cytology* Animals Cell Death / drug effects Cell Differentiation / drug effects Female Flow Cytometry Fluorescence Injections, Intravenous Intracellular Space / drug effects, metabolism Mice Mice, Inbred C57BL Molecular Imaging / methods* Oligopeptides / pharmacology*, toxicity Pinocytosis / drug effects Quantum Dots* Staining and Labeling* Stem Cells / cytology*, drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Oligopeptides; 0/octa-arginine peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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