Document Detail


Quantum dot labeling based on near-field optical imaging of CD44 molecules.
MedLine Citation:
PMID:  19959369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lateral organization of membrane proteins and lipids domains has a direct impact on many cellular processes, but generally these domains are too small to be resolved by diffraction-limited resolution of fluorescence microscopy. Here, we use quantum dot (QD) labeling based on near-field optical imaging, to study the nanoscale organization of hyaluronan receptor CD44 molecules of fixed mesenchymal stem cells (MSCs) in air, with a optical resolution down to 50 nm. The photostability and high luminance of QD evidently improve the signal-to-noise ratio and reproducibility of near-field optical data. Importantly, the blinking-intensity analysis was proposed to identify single QD, providing a calibration to relate intensity to numbers of antibody for the first time. Additionally, the fluorescence-topographic imaging enables us to investigate the topographic location pattern. Our results demonstrate that CD44 molecules on MSCs are enriched into nanosized domain and they predominantly locate on the peak of the membrane protrusions, which may contribute to clarify the underlying mechanism of functions ascribed to these molecules.
Authors:
Jianan Chen; Yin Pei; Zhengwei Chen; Jiye Cai
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-11-11
Journal Detail:
Title:  Micron (Oxford, England : 1993)     Volume:  41     ISSN:  1878-4291     ISO Abbreviation:  Micron     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312850     Medline TA:  Micron     Country:  England    
Other Details:
Languages:  eng     Pagination:  198-202     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Chemistry, Jinan University, Guangzhou 510632, China.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD44 / analysis*
Cell Membrane / chemistry*
Humans
Mesenchymal Stem Cells / chemistry*
Microscopy / methods*
Quantum Dots*
Staining and Labeling / methods*
Grant Support
ID/Acronym/Agency:
R01 HL60635/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD44

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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