| Quantum dot-based fluorescence resonance energy transfer with improved FRET efficiency in capillary flows. | |
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MedLine Citation:
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PMID: 16878892 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence resonance energy transfer (FRET)-based nanosensors with quantum dots (QDs) as donors and organic dyes as acceptors have long been of interest for the detection of biomolecules such as nucleic acids, but their low FRET efficiency in bulk solution has prevented the sensitive detection of nucleic acids due to the large size of the QDs and the long length of nucleic acids. Here we describe a novel approach to improve the detection sensitivity of QD-based nanosensors using single-molecule detection in a capillary flow. In comparison with bulk measurement, single-molecule detection in a capillary flow possesses the unique advantages of improved FRET efficiency, high sensitivity, prevention of photobleaching, and low sample consumption. Greater FRET efficiency was obtained due to the deformation of DNA in the capillary stream. This technique can be easily extended to sensitive bimolecular analysis in microfluidic chips, and it may also offer a promising approach to study the deformation of small nucleic acids in fluid flow. |
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Authors:
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Chun-Yang Zhang; Lawrence W Johnson |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Analytical chemistry Volume: 78 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-01 Completed Date: 2007-06-04 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5532-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry, York College and The Graduate Center, The City University of New York, Jamaica, New York 11451, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis Electrodes Fluorescence Resonance Energy Transfer* Microfluidics* Oligonucleotides / analysis Quantum Dots* Sensitivity and Specificity Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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GM08153/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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