| Quantum yields and kinetics of the firefly bioluminescence reaction of beetle luciferases. | |
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MedLine Citation:
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PMID: 20663080 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). Mutant analyses of active site-substituted Pyrocoelia miyako luciferases showed that, although k(cat) was decreased by the mutations, the QY was not significantly affected. |
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Authors:
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Kazuki Niwa; Yoshiro Ichino; Shiho Kumata; Yoshihiro Nakajima; Yoshihiro Hiraishi; Dai-Ichiro Kato; Vadim R Viviani; Yoshihiro Ohmiya |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 86 ISSN: 1751-1097 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 1046-9 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal Compilation. The American Society of Photobiology. |
Affiliation:
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National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology(AIST), Tsukuba, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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